ArticleMolecular and cellular biology2004
Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3' processing.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Diverse and conserved roles of the protein Ssu72 in eukaryotes: from yeast to higher organisms.Current genetics · 2021Review
- Gene-Specific Control of tRNA Expression by RNA Polymerase II.Molecular cell · 2020Article
- The Integrator complex regulates differential snRNA processing and fate of adult stem cells in the highly regenerative planarian Schmidtea mediterranea.PLoS genetics · 2018Article
- The little elongation complex functions at initiation and elongation phases of snRNA gene transcription.Molecular cell · 2013Article
- The RNA polymerase II CTD coordinates transcription and RNA processing.Genes & development · 2012Review
- In vitro RNase and nucleic acid binding activities implicate coilin in U snRNA processing.PloS one · 2012Article
- A primate herpesvirus uses the integrator complex to generate viral microRNAs.Molecular cell · 2011Article
- Updating the CTD Story: From Tail to Epic.Genetics research international · 2011Article
- Genetic organization, length conservation, and evolution of RNA polymerase II carboxyl-terminal domain.Molecular biology and evolution · 2010Article
- The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain.The Journal of biological chemistry · 2010Article
- HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response.The Journal of biological chemistry · 2009Article
- Transcription termination by nuclear RNA polymerases.Genes & development · 2009Article
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- Human U2 snRNA genes exhibit a persistently open transcriptional state and promoter disassembly at metaphase.Molecular and cellular biology · 2008Article
- Transcriptional regulation of human small nuclear RNA genes.Biochimica et biophysica acta · 2008Review
- Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression.Science (New York, N.Y.) · 2007Article
- Review
- Review
- P-TEFb is not an essential elongation factor for the intronless human U2 snRNA and histone H2b genes.The EMBO journal · 2005Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
U small nuclear RNAs (snRNAs) and mRNAs are both transcribed by RNA polymerase II (Pol II), but the snRNAs have unusual TATA-less promoters and are neither spliced nor polyadenylated; instead, 3' processing is directed by a highly conserved 3' end formation signal that requires initiation from an snRNA promoter. Here we show that the C-terminal domain (CTD) of Pol II is required for efficient U2 snRNA transcription, as it is for mRNA transcription. However, CTD kinase inhibitors, such as 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) and 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H7), that block mRNA elongation do not affect U2 transcription, although 3' processing of the U2 primary transcript is impaired. We show further that U2 transcription is preferentially inhibited by low doses of UV irradiation or actinomycin D, which induce CTD kinase activity, and that UV inhibition can be rescued by treatment with DRB or H7. We propose that Pol II complexes transcribing snRNAs and mRNAs have distinct CTD phosphorylation patterns. mRNA promoters recruit factors including kinases that hyperphosphorylate the CTD, and the CTD in turn recruits proteins needed for mRNA splicing and polyadenylation. We predict that snRNA promoters recruit factors including a CTD kinase(s) whose snRNA-specific phosphorylation pattern recruits factors required for promoter-coupled 3' end formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.