ArticleMolecular and cellular biology2004
The origin recognition complex and Sir4 protein recruit Sir1p to yeast silent chromatin through independent interactions requiring a common Sir1p domain.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 70 citations in OpenAlex.
- Molecular Mechanisms of Transcription Factors with Dual Activator and Repressor Functions.Molecular and cellular biology · 2026Review
- Tissue-Specific DNA Replication Defects inGenetics · 2020Article
- Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 inGenetics · 2019Article
- Nonreplicative functions of the origin recognition complex.Nucleus (Austin, Tex.) · 2018Review
- Variants of the Sir4 Coiled-Coil Domain Improve Binding to Sir3 for Heterochromatin Formation inG3 (Bethesda, Md.) · 2017Article
- Mechanisms and regulation of DNA replication initiation in eukaryotes.Critical reviews in biochemistry and molecular biology · 2017Review
- Identification of the Target of the Retrograde Response that Mediates Replicative Lifespan Extension in Saccharomyces cerevisiae.Genetics · 2016Article
- Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome.Nucleic acids research · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Evolution and Functional Trajectory of Sir1 in Gene Silencing.Molecular and cellular biology · 2016Article
- Sirtuins in epigenetic regulation.Chemical reviews · 2015Review
- Structural basis for allosteric stimulation of Sir2 activity by Sir4 binding.Genes & development · 2013Article
- The SUMO E3 ligase Siz2 exerts a locus-dependent effect on gene silencing in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Reinventing heterochromatin in budding yeasts: Sir2 and the origin recognition complex take center stage.Eukaryotic cell · 2011Review
- Palmitoylation controls the dynamics of budding-yeast heterochromatin via the telomere-binding protein Rif1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Co-evolution of transcriptional silencing proteins and the DNA elements specifying their assembly.PLoS biology · 2010Article
- Proliferating cell nuclear antigen (PCNA) is required for cell cycle-regulated silent chromatin on replicated and nonreplicated genes.The Journal of biological chemistry · 2010Article
- The conserved bromo-adjacent homology domain of yeast Orc1 functions in the selection of DNA replication origins within chromatin.Genes & development · 2010Article
- Expanded roles of the origin recognition complex in the architecture and function of silenced chromatin in Saccharomyces cerevisiae.Molecular and cellular biology · 2010Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
Sir1p is one of four SIR (silent information regulator) proteins required for silencing the cryptic mating-type locus HMRa in the budding yeast Saccharomyces cerevisiae. A Sir1p interaction with Orc1p, the largest subunit of the origin recognition complex (ORC), is critical for Sir1p's ability to bind HMRa and function in the formation of silent chromatin. Here we show that a discrete domain within Sir1p, the ORC interaction region (OIR), was necessary and sufficient for a Sir1p-ORC interaction. The OIR contains the originally defined silencer recognition-defective region as well as additional amino acids. In addition, a Sir1p-Sir4p interaction required a larger region of Sir1p that included the OIR. Amino acid substitutions causing defects in either a Sir1p-Orc1p or a Sir1p-Sir4p interaction reduced HMRa silencing and Sir1p binding to HMRa in chromatin. These data support a model in which Sir1p's association with HMRa is mediated by separable Sir1p-ORC and Sir1p-Sir4p interactions requiring a common Sir1p domain, and they indicate that a Sir1p-ORC interaction is restricted to silencers, at least in part, through interactions with Sir4p.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.