ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2004
Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Performance evaluation of the BioFire respiratory panel 2.1 plus and exploratory analysis of Ct values for respiratory pathogen quantification.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Diagnosis of COVID-19, vitality of emerging technologies and preventive measures.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Simple, Inexpensive RNA Isolation and One-Step RT-qPCR Methods for SARS-CoV-2 Detection and General Use.Current protocols · 2021Article
- Crosstalk Between Covid-19 and Associated Neurological Disorders: A Review.Current neuropharmacology · 2021Review
- Laboratory diagnosis of coronavirus disease-2019 (COVID-19).Clinica chimica acta; international journal of clinical chemistry · 2020Review
- A Narrative Review of COVID-19: The New Pandemic Disease.Iranian journal of medical sciences · 2020Review
- A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2.Heliyon · 2020Article
- COVID-19 pandemic: an overview of epidemiology, pathogenesis, diagnostics and potential vaccines and therapeutics.Therapeutic delivery · 2020Review
- Potential Rapid Diagnostics, Vaccine and Therapeutics for 2019 Novel Coronavirus (2019-nCoV): A Systematic Review.Journal of clinical medicine · 2020Review
- Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins.Biochemical and biophysical research communications · 2014Article
- Diagnostics of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid antigen using chicken immunoglobulin Y.Poultry science · 2012Article
- Rapid and Real-time Detection of Human Viral Infections: Current Trends and Future Perspectives.Proceedings of the National Academy of Sciences, India. Section B · 2012Article
- Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses.Archives of virology · 2011Article
- Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in human serum using a localized surface plasmon coupled fluorescence fiber-optic biosensor.Biosensors & bioelectronics · 2009Article
- Rapid and real-time detection technologies for emerging viruses of biomedical importance.Journal of biosciences · 2008Article
- Highly sensitive methods based on seminested real-time reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 unspliced and multiply spliced RNA and proviral DNA.Journal of clinical microbiology · 2008Article
- Molecular targets for diagnostics and therapeutics of severe acute respiratory syndrome (SARS-CoV).Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques · 2008Article
- Isolation and identification of an scFv antibody against nucleocapsid protein of SARS-CoV.Microbes and infection · 2007Article
- Detection of feline coronavirus using microcantilever sensors.Measurement science & technology · 2006Article
- Identifying epitopes responsible for neutralizing antibody and DC-SIGN binding on the spike glycoprotein of the severe acute respiratory syndrome coronavirus.Journal of virology · 2006Article
Corrections and comments
- Commented on by
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by our method were not detected by single-round, real-time RT-PCR, which suggests that real-time nested PCR has the potential for increased sensitivity, leading to earlier detection of SARS.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.