ArticleMolecular biology of the cell2004
Routing of Hansenula polymorpha alcohol oxidase: an alternative peroxisomal protein-sorting machinery.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 76 citations in OpenAlex.
- Peroxisome Carrier SLC25A17: Potential Biomarker for Peroxisome Dysfunction and Human Disease.International journal of molecular sciences · 2026Review
- The peroxisomal importomer can accommodate an intrinsically disordered protein of 1247 residues.Histochemistry and cell biology · 2026Article
- Towards solving the mystery of peroxisomal matrix protein import.Trends in cell biology · 2024Review
- Article
- Engineering the methylotrophic yeastFrontiers in bioengineering and biotechnology · 2023Article
- Synthetic methylotrophic yeasts for the sustainable fuel and chemical production.Biotechnology for biofuels and bioproducts · 2022Review
- PEX5 translocation into and out of peroxisomes drives matrix protein import.Molecular cell · 2022Article
- Insights into the Structure and Function of the Pex1/Pex6 AAA-ATPase in Peroxisome Homeostasis.Cells · 2022Review
- Two Pex5 Proteins With Different Cargo Specificity Are Critical for Peroxisome Function in Ustilago maydis.Frontiers in cell and developmental biology · 2022Article
- Peroxisomal Cofactor Transport.Biomolecules · 2020Review
- The Peroxisomal Targeting Signal 3 (PTS3) of the Budding Yeast Acyl-CoA Oxidase Is a Signal Patch.Frontiers in cell and developmental biology · 2020Article
- The autophagic degradation of cytosolic pools of peroxisomal proteins by a new selective pathway.Autophagy · 2020Article
- Hansenula polymorpha Aat2p is targeted to peroxisomes via a novel Pex20p-dependent pathway.FEBS letters · 2018Article
- Crosstalk between mitochondria and peroxisomes.World journal of biological chemistry · 2015Review
- A PEX7-centered perspective on the peroxisomal targeting signal type 2-mediated protein import pathway.Molecular and cellular biology · 2014Article
- Dual targeting of peroxisomal proteins.Frontiers in physiology · 2013Review
- A cargo-centered perspective on the PEX5 receptor-mediated peroxisomal protein import pathway.The Journal of biological chemistry · 2013Article
- Import of proteins into the peroxisomal matrix.Frontiers in physiology · 2013Review
- Lipid droplets and peroxisomes: key players in cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down.Genetics · 2013Review
- D-amino acid-induced expression of D-amino acid oxidase in the yeast Schizosaccharomyces pombe.Current microbiology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Import of Hansenula polymorpha alcohol oxidase (AO) into peroxisomes is dependent on the PTS1 receptor, HpPex5p. The PTS1 of AO (-LARF) is sufficient to direct reporter proteins to peroxisomes. To study AO sorting in more detail, strains producing mutant AO proteins were constructed. AO containing a mutation in the FAD binding fold was mislocalized to the cytosol. This indicates that the PTS1 of AO is not sufficient for import of AO. AO protein in which the PTS1 was destroyed (-LARA) was normally sorted to peroxisomes. Moreover, C-terminal deletions of up to 16 amino acids did not significantly affect AO import, indicating that the PTS1 was not necessary for targeting. Consistent with these observations we found that AO import occurred independent from the C-terminal TPR-domain of HpPex5p, known to bind PTS1 peptides. Synthesis of the N-terminal domain (amino acids 1-272) of HpPex5p in pex5 cells restored AO import, whereas other PTS1 proteins were mislocalized to the cytosol. These data indicate that AO is imported via a novel HpPex5p-dependent protein translocation pathway, which does not require the PTS1 of AO and the C-terminal TPR domains of HpPex5p, but involves FAD binding and the N-terminus of HpPex5p.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.