ArticleMolecular biology of the cell2004
ago1 and dcr1, two core components of the RNA interference pathway, functionally diverge from rdp1 in regulating cell cycle events in Schizosaccharomyces pombe.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Ago1 is required for the regulation of mitochondrial translation under heat stress in Schizosaccharomyces pombe.The Journal of biological chemistry · 2026Article
- Transcription-replication conflict resolution by nuclear RNA interference.Molecular cell · 2025Article
- Lessons on fruiting body morphogenesis from genomes and transcriptomes ofStudies in mycology · 2023Article
- Spotting the Targets of the Apospory ControllerPlants (Basel, Switzerland) · 2022Article
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- The contribution of non-essentialOpen biology · 2018Article
- Genome-Wide Analysis of DCL, AGO, and RDR Gene Families in Pepper (Capsicum Annuum L.).International journal of molecular sciences · 2018Article
- Regulation of transcriptional silencing and chromodomain protein localization at centromeric heterochromatin by histone H3 tyrosine 41 phosphorylation in fission yeast.Nucleic acids research · 2018Article
- Mutations disrupting histone methylation have different effects on replication timing in S. pombe centromere.PloS one · 2013Article
- A distinct expression pattern of the long 3'-untranslated region dicer mRNA and its implications for posttranscriptional regulation in colorectal cancer.Clinical and translational gastroenterology · 2012Article
- Autoregulation of convergent RNAi genes in fission yeast.Genes & development · 2011Article
- RNAi genes pave their own way.Genes & development · 2011Article
- Characterizing the role of RNA silencing components in Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2010Article
- Overexpression of dicer as a result of reduced let-7 MicroRNA levels contributes to increased cell proliferation of oral cancer cells.Genes, chromosomes & cancer · 2010Article
- Effect of betulinic acid on the regulation of Hiwi and cyclin B1 in human gastric adenocarcinoma AGS cells.Acta pharmacologica Sinica · 2010Article
- Decreased Dicer expression elicits DNA damage and up-regulation of MICA and MICB.The Journal of cell biology · 2008Article
- DICER1 and PRKRA in Colon Adenocarcinoma.Biomarker insights · 2008Article
- RNA interference guides histone modification during the S phase of chromosomal replication.Current biology : CB · 2008Article
- RNA silencing gene truncation in the filamentous fungus Aspergillus nidulans.Eukaryotic cell · 2008Article
- Up-regulation of dicer, a component of the MicroRNA machinery, in prostate adenocarcinoma.The American journal of pathology · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the fission yeast Schizosaccharomyces pombe, three genes that function in the RNA interference (RNAi) pathway, ago1+, dcr1+, and rdp1+, have recently been shown to be important for timely formation of heterochromatin and accurate chromosome segregation. In the present study, we present evidence that null mutants for ago1+ and dcr1+ but not rdp1+, exhibit abnormal cytokinesis, cell cycle arrest deficiencies, and mating defects. Subsequent analyses showed that ago1+ and dcr1+ are required for regulated hyperphosphorylation of Cdc2 when encountering genotoxic insults. Because rdp1+ is dispensable for this process, the functions of ago1+ and dcr1+ in this pathway are presumably independent of their roles in RNAi-mediated heterochromatin formation and chromosome segregation. This was further supported by the finding that ago1+ is a multicopy suppressor of the S-M checkpoint deficiency and cytokinesis defects associated with loss of Dcr1 function, but not for the chromosome segregation defects of this mutant. Accordingly, we conclude that Dcr1-dependent production of small interfering RNAs is not required for enactment and/or maintenance of certain cell cycle checkpoints and that Ago1 and Dcr1 functionally diverge from Rdp1 to control cell cycle events in fission yeast. Finally, exogenous expression of hGERp95/EIF2C2/hAgo2, a human Ago1 homolog implicated in posttranscriptional gene silencing, compensated for the loss of ago1+ function in S. pombe. This suggests that PPD proteins may also be important for regulation of cell cycle events in higher eukaryotes.
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