ArticleProceedings of the National Academy of Sciences of the United States of America2004
The chitinolytic cascade in Vibrios is regulated by chitin oligosaccharides and a two-component chitin catabolic sensor/kinase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 107 papers.
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Who cites it
107 citing papers in PubMed, 247 citations in OpenAlex.
- H-NOX and NosP Regulate Flagellar Protein and Virulence Factor Production in Vibrio Cholerae.Molecular & cellular proteomics : MCP · 2026Article
- Structure-based classification of bacterial chitooligosaccharide-specific outer membrane porins (ChiPs).The Journal of biological chemistry · 2026Article
- Additional Binding Domain of Marine Chitinase Increases Binding Specificity and Prolongs the Lifetime of Productive Binding State.Biochemistry · 2026Article
- A conserved, immune-regulated peritrophin promotes Vibrio cholerae colonization of the arthropod intestine.Nature communications · 2026Article
- Characterization of an Atypical GH19 Family Chitinase fromMicroorganisms · 2026Article
- Genome-based characterization of AHPND and non-AHPNDFrontiers in microbiology · 2026Article
- Structural modeling reveals the allosteric switch controlling the chitin utilization program ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- ChiVariARIBA: a modular, editable workflow and database for characterising chitin gene variation inMicrobial genomics · 2025Article
- Environmental DNA adsorption to chitin can promote horizontal gene transfer by natural transformation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Two transmembrane transcriptional regulators coordinate to activate chitin-induced natural transformation in Vibrio cholerae.PLoS genetics · 2025Article
- Complete xylan utilization pathway and regulation mechanisms involved in marine algae degradation by cosmopolitan marine and human gut microbiota.The ISME journal · 2025Article
- Diversity and Potential Metabolic Characteristics of Culturable Copiotrophic Bacteria That Can Grow on Low-Nutrient Medium in Zhenbei Seamount in the South China Sea.Microbial ecology · 2024Article
- Response Surface Methodology-Based Optimization of the Chitinolytic Activity ofMicroorganisms · 2024Article
- Bacteria face trade-offs in the decomposition of complex biopolymers.PLoS computational biology · 2024Article
- Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation.Scientific reports · 2023Article
- Chitin degradation by Synechococcus WH7803.Scientific reports · 2023Article
- Inherited chitinases enable sustained growth and rapid dispersal of bacteria from chitin particles.Nature microbiology · 2023Article
- Article
- Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.Microbiology (Reading, England) · 2023Review
47 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
Abstract
Chitin, a highly insoluble polymer of GlcNAc, is produced in massive quantities in the marine environment. Fortunately for survival of aquatic ecosystems, chitin is rapidly catabolized by marine bacteria. Here we describe a bacterial two-component hybrid sensor/kinase (of the ArcB type) that rigorously controls expression of approximately 50 genes, many involved in chitin degradation. The sensor gene, chiS, was identified in Vibrio furnissii and Vibrio cholerae (predicted amino acid sequences, full-length: 84% identical, 93% similar). Mutants of chiS grew normally on GlcNAc but did not express extracellular chitinase, a specific chitoporin, or beta-hexosaminidases, nor did they exhibit chemotaxis, transport, or growth on chitin oligosaccharides such as (GlcNAc)(2). Expression of these systems requires three components: wild-type chiS; a periplasmic high-affinity chitin oligosaccharide, (GlcNAc)(n) (n > 1), binding protein (CBP); and the environmental signal, (GlcNAc)(n). Our data are consistent with the following model. In the uninduced state, CBP binds to the periplasmic domain of ChiS and "locks" it into the minus conformation. The environmental signal, (GlcNAc)(n), dissociates the complex by binding to CBP, releasing ChiS, yielding the plus phenotype (expression of chitinolytic genes). In V. cholerae, a cluster of 10 contiguous genes (VC0620-VC0611) apparently comprise a (GlcNAc)(2) catabolic operon. CBP is encoded by the first, VC0620, whereas VC0619-VC0616 encode a (GlcNAc)(2) ABC-type permease. Regulation of chiS requires expression of CBP but not (GlcNAc)(2) transport. (GlcNAc)(n) is suggested to be essential for signaling these cells that chitin is in the microenvironment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.