ArticleWorld journal of gastroenterology2004
Transfection of mEpo gene to intestinal epithelium in vivo mediated by oral delivery of chitosan-DNA nanoparticles.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 84 citations in OpenAlex.
- Implant Renal Injury-Responsive Cells to Supplement Erythropoietin and Protect Kidney Injury.MedComm · 2025Article
- Thermoresponsive Bacteriophage Nanocarrier as a Gene Delivery Vector Targeted to the Gastrointestinal Tract.Molecular therapy. Nucleic acids · 2018Article
- Chitosans for delivery of nucleic acids.Advanced drug delivery reviews · 2013Review
- In vitro and in vivo models for the study of oral delivery of nanoparticles.Advanced drug delivery reviews · 2013Review
- Oral gene application using chitosan-DNA nanoparticles induces transferable tolerance.Clinical and vaccine immunology : CVI · 2012Article
- Preparation, characterization and transfection efficacy of chitosan nanoparticles containing the intestinal trefoil factor gene.Molecular biology reports · 2012Article
- Chitosan-plasmid nanoparticle formulations for IM and SC delivery of recombinant FGF-2 and PDGF-BB or generation of antibodies.Gene therapy · 2009Article
- Composite hydrogel formulations of stratifin to control MMP-1 expression in dermal fibroblasts.Pharmaceutical research · 2009Article
- Gene transfer to hemophilia A mice via oral delivery of FVIII-chitosan nanoparticles.Journal of controlled release : official journal of the Controlled Release Society · 2008Article
- Past, present, and future technologies for oral delivery of therapeutic proteins.Journal of pharmaceutical sciences · 2008Review
- Prevention of airway inflammation with topical cream containing imiquimod and small interfering RNA for natriuretic peptide receptor.Genetic vaccines and therapy · 2008Article
- Highly stomach-selective gene transfer following gastric serosal surface instillation of naked plasmid DNA in rats.Journal of gastroenterology · 2008Article
- New methodologies to characterize the effectiveness of the gene transfer mediated by DNA-chitosan nanoparticles.International journal of nanomedicine · 2008Article
- Chitosan nanoparticles for oral drug and gene delivery.International journal of nanomedicine · 2006Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo prepare the chitosan-pmEpo nanoparticles and to study their ability for transcellular and paracellular transport across intestinal epithelia by oral administration.
methodsICR mice were fed with recombinant plasmid AAV-tetO-CMV-mEpo (containing mEpo gene) or pCMVbeta(containing LacZ gene), whether it was wrapped by chitosan or no. Its size and shape were observed by transmission electron microscopy. Agarose gel electrophoresis was used to assess the efficiency of encapsulation and stability against nuclease digestion. Before and after oral treatmant, blood samples were collected by retro-orbital puncture, and hematocrits were used to show the physiological effect of mEpo.
resultsChitosan was able to successfully wrap the plasmid and to protect it from DNase degradation. Transmission electron microscopy showed that freshly prepared particles were approximately 70-150 nm in size and fairly spherical. Three days after fed the chitosan-pCMVbeta complex was fed, the mice were killed and most of the stomach and 30% of the small intestine were stained. Hematocrit was not modified in naive and 'naked' mEpo-fed mice, a rapid increase of hematocrit was observed during the first 4 days of treatment in chitosan-mEpo-fed animals, reaching 60.9+/-1.2% (P<0.01), and sustained for a week. The second feed (6 days after the first feed) was still able to promote a second hematocrit increase in chitosan-mEpo-fed animals, reaching 65.9+/-1.4% (P<0.01), while the second hematocrit increase did not appear in the 'naked' mEpo-second-fed mice.
conclusionOral chitosan-DNA nanoparticles can efficiently deliver genes to enterocytes, and may be used as a useful tool for gene transfer.
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