ArticleBiophysical journal2004
Spatial analysis of 3' phosphoinositide signaling in living fibroblasts: II. Parameter estimates for individual cells from experiments.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 34 citations in OpenAlex.
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- A phase field model with stochastic input simulates cellular gradient sensing, morphodynamics, and fidelity of haptotaxis.bioRxiv : the preprint server for biology · 2026Article
- PLEKHG3 enhances polarized cell migration by activating actin filaments at the cell front.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling.The Journal of cell biology · 2015Article
- Live-cell fluorescence microscopy with molecular biosensors: what are we really measuring?Biophysical journal · 2012Article
- Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism.The Journal of cell biology · 2012Article
- How cells integrate complex stimuli: the effect of feedback from phosphoinositides and cell shape on cell polarization and motility.PLoS computational biology · 2012Article
- In chemotaxing fibroblasts, both high-fidelity and weakly biased cell movements track the localization of PI3K signaling.Biophysical journal · 2011Article
- A comparison of mathematical models for polarization of single eukaryotic cells in response to guided cues.PLoS computational biology · 2011Review
- Computational analysis of the spatiotemporal coordination of polarized PI3K and Rac1 activities in micro-patterned live cells.PloS one · 2011Article
- Systematic quantification of negative feedback mechanisms in the extracellular signal-regulated kinase (ERK) signaling network.The Journal of biological chemistry · 2010Article
- Directional persistence of cell migration coincides with stability of asymmetric intracellular signaling.Biophysical journal · 2010Article
- Spontaneous phosphoinositide 3-kinase signaling dynamics drive spreading and random migration of fibroblasts.Journal of cell science · 2009Article
- Modelling cellular signalling systems.Essays in biochemistry · 2008Review
- Imaging and manipulating phosphoinositides in living cells.The Journal of physiology · 2007Article
- Phosphoinositides and Rho proteins spatially regulate actin polymerization to initiate and maintain directed movement in a one-dimensional model of a motile cell.Biophysical journal · 2007Article
- Deterministic model of dermal wound invasion incorporating receptor-mediated signal transduction and spatial gradient sensing.Biophysical journal · 2006Article
- Quantitative model of Ras-phosphoinositide 3-kinase signalling cross-talk based on co-operative molecular assembly.The Biochemical journal · 2006Article
- Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts.The Journal of cell biology · 2005Article
- Distinguishing modes of eukaryotic gradient sensing.Biophysical journal · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fibroblast migration is directed by gradients of platelet-derived growth factor (PDGF) during wound healing. As in other chemotactic systems, it has been shown recently that localized stimulation of intracellular phosphoinositide (PI) 3-kinase activity and production of 3' PI lipids in the plasma membrane are important events in the signaling of spatially biased motility processes. In turn, 3' PI localization depends on the effective diffusion coefficient, D, and turnover rate constant, k, of these lipids. Here we present a systematic and direct comparison of mathematical model calculations and experimental measurements to estimate the values of the effective 3' PI diffusion coefficient, D, turnover rate constant, k, and other parameters in individual fibroblasts stimulated uniformly with PDGF. In the context of our uniform stimulation model, the values of D and k in each cell were typically estimated within 10-20% or less, and the mean values across all of the cells analyzed were D = 0.37 +/- 0.25 microm2/s and k = 1.18 +/- 0.54 min(-1). In addition, we report that 3' PI turnover is not affected by PDGF receptor signaling in our cells, allowing us to focus our attention on the regulation of 3' PI production as this system is studied further.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.