ArticleBiophysical journal2004
Spatial analysis of 3' phosphoinositide signaling in living fibroblasts: I. Uniform stimulation model and bounds on dimensionless groups.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Live-cell fluorescence microscopy with molecular biosensors: what are we really measuring?Biophysical journal · 2012Article
- Mathematical analysis of steady-state solutions in compartment and continuum models of cell polarization.Mathematical biosciences and engineering : MBE · 2011Article
- A comparison of mathematical models for polarization of single eukaryotic cells in response to guided cues.PLoS computational biology · 2011Review
- Computational analysis of the spatiotemporal coordination of polarized PI3K and Rac1 activities in micro-patterned live cells.PloS one · 2011Article
- Modeling robustness tradeoffs in yeast cell polarization induced by spatial gradients.PloS one · 2008Article
- Wave-pinning and cell polarity from a bistable reaction-diffusion system.Biophysical journal · 2008Article
- Modelling cellular signalling systems.Essays in biochemistry · 2008Review
- Phosphoinositides and Rho proteins spatially regulate actin polymerization to initiate and maintain directed movement in a one-dimensional model of a motile cell.Biophysical journal · 2007Article
- Deterministic model of dermal wound invasion incorporating receptor-mediated signal transduction and spatial gradient sensing.Biophysical journal · 2006Article
- Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts.The Journal of cell biology · 2005Article
- Distinguishing modes of eukaryotic gradient sensing.Biophysical journal · 2005Article
- Spatial analysis of 3' phosphoinositide signaling in living fibroblasts, III: influence of cell morphology and morphological Polarity.Biophysical journal · 2005Article
- Computational approaches for modeling regulatory cellular networks.Trends in cell biology · 2004Review
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2 authors.
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Abstract
Fluorescent protein probes now permit spatial distributions of specific intracellular signaling molecules to be observed in real time. Mathematical models have been used to simulate molecular gradients and other spatial patterns within cells, and the output of such models may be compared directly with experiments if the binding of the fluorescent probe and the physics of the imaging technique are each incorporated. Here we present a comprehensive model describing the dynamics of 3' phosphoinositides (PIs), lipid second messengers produced in the plasma membrane in response to stimulation of the PI 3-kinase signaling pathway, as monitored in the cell-substratum contact area using total internal reflection fluorescence microscopy. With this technique it was previously shown that uniform stimulation of fibroblasts with platelet-derived growth factor elicits the formation of axisymmetric 3' PI gradients, which we now characterize in the context of our model. We find that upper and lower bounds on the relevant dimensionless model parameter values for an individual cell can be calculated from four well-defined fluorescence measurements. Based on our analysis, we expect that the key dimensionless group, the ratio of 3' PI turnover and diffusion rates, can be estimated within approximately 20% or less.
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