ArticleBiophysical journal2004
A lipid-specific toxin reveals heterogeneity of sphingomyelin-containing membranes.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 141 citations in OpenAlex.
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- Pharmacological effects of bioactive agents in earthworm extract: A comprehensive review.Animal models and experimental medicine · 2024Review
- A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane.Scientific reports · 2024Article
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- HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.Nature communications · 2023Article
- PGP-14 establishes a polar lipid permeability barrier within the C. elegans pharyngeal cuticle.PLoS genetics · 2023Article
- The C10orf76-PI4KB axis orchestrates CERT-mediated ceramide trafficking to the distal Golgi.The Journal of cell biology · 2023Article
- Cell density-dependent membrane distribution of ganglioside GM3 in melanoma cells.Cellular and molecular life sciences : CMLS · 2023Article
- A single point mutation expands the applicability of ostreolysin A6 in biomedicine.Scientific reports · 2023Article
- Compartmentalization of Sphingolipid metabolism: Implications for signaling and therapy.Pharmacology & therapeutics · 2022Review
- Beyond pore formation: reorganization of the plasma membrane induced by pore-forming proteins.Cellular and molecular life sciences : CMLS · 2021Review
- How Does Liquid-Liquid Phase Separation in Model Membranes Reflect Cell Membrane Heterogeneity?Membranes · 2021Review
- The marginal cells of the Caenorhabditis elegans pharynx scavenge cholesterol and other hydrophobic small molecules.Nature communications · 2019Article
- 2-Hydroxy-oleic acid does not activate sphingomyelin synthase activity.The Journal of biological chemistry · 2018Article
- Molecular mechanism of pore formation by aerolysin-like proteins.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Staurosporines decrease ORMDL proteins and enhance sphingomyelin synthesis resulting in depletion of plasmalemmal phosphatidylserine.Scientific reports · 2016Article
- Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow.Molecular biology of the cell · 2016Article
- Comparative lipid analysis in the normal and cancerous organoids of MDCK cells.Journal of biochemistry · 2016Article
- Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assembly.Nature communications · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the heterogenous organization of lipids in biological membranes. Sphingomyelin (SM) is a major plasma membrane lipid that forms lipid domains together with cholesterol and glycolipids. Using SM-specific toxin, lysenin, we showed that in cultured epithelial cells the accessibility of the toxin to SM is different between apical and basolateral membranes. Apical membranes are highly enriched with glycolipids. The inhibitory role of glycolipids in the binding of lysenin to SM was confirmed by comparing the glycolipid-deficient mutant melanoma cell line with its parent cell. Model membrane experiments indicated that glycolipid altered the local density of SM so that the affinity of the lipid for lysenin was decreased. Our results indicate that lysenin recognizes the heterogenous organization of SM in biomembranes and that the organization of SM differs between different cell types and between different membrane domains within the same cell. Isothermal titration calorimetry suggests that lysenin binding to SM is presumably the result of a SM-lysenin complex formation of specific stoichiometry, thus supporting the idea of the existence of small condensed lipid complexes consisting of just a few lipid molecules in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.