ArticleBiophysical journal2004
Glucose accumulation can account for the initial water flux triggered by Na+/glucose cotransport.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Glucose transporters in the small intestine in health and disease.Pflugers Archiv : European journal of physiology · 2020Review
- Article
- The Sodium Glucose Cotransporter SGLT1 Is an Extremely Efficient Facilitator of Passive Water Transport.The Journal of biological chemistry · 2016Article
- Structural determinants of water permeation through the sodium-galactose transporter vSGLT.Biophysical journal · 2014Article
- Transient formation of water-conducting states in membrane transporters.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Cotransport of water by Na⁺-K⁺-2Cl⁻ cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2.The Journal of physiology · 2012Article
- Osmoregulation and epithelial water transport: lessons from the intestine of marine teleost fish.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2012Review
- The structural pathway for water permeation through sodium-glucose cotransporters.Biophysical journal · 2011Article
- Routes of epithelial water flow: aquaporins versus cotransporters.Biophysical journal · 2010Article
- Water permeation through the sodium-dependent galactose cotransporter vSGLT.Biophysical journal · 2010Article
- Water-transporting proteins.The Journal of membrane biology · 2010Review
- Osmotic water transport with glucose in GLUT2 and SGLT.Biophysical journal · 2008Article
- From membrane pores to aquaporins: 50 years measuring water fluxes.Journal of biological physics · 2007Article
- The mechanism of water transport in Na+-coupled glucose transporters expressed in Xenopus oocytes.Biophysical journal · 2007Article
- Voltage-clamp fluorometry in the local environment of the C255-C511 disulfide bridge of the Na+/glucose cotransporter.Biophysical journal · 2007Article
- Water transport by GLUT2 expressed in Xenopus laevis oocytes.The Journal of physiology · 2007Article
- Intracellular hypertonicity is responsible for water flux associated with Na+/glucose cotransport.Biophysical journal · 2006Article
- Identification of a disulfide bridge linking the fourth and the seventh extracellular loops of the Na+/glucose cotransporter.The Journal of general physiology · 2006Article
- Water transport by Na+-coupled cotransporters of glucose (SGLT1) and of iodide (NIS). The dependence of substrate size studied at high resolution.The Journal of physiology · 2006Article
- Water permeability of Na+-K+-2Cl- cotransporters in mammalian epithelial cells.The Journal of physiology · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the last decade, several cotransport studies have led to the proposal of secondary active transport of water, challenging the dogma that all water transport is passive. The major observation leading to this interpretation was that a Na+ influx failed to reproduce the large and rapid cell swelling induced by Na+/solute cotransport. We have investigated this phenomenon by comparing a Na+/glucose (hSGLT1) induced water flux to water fluxes triggered either by a cationic inward current (using ROMK2 K+ channels) or by a glucose influx (using GLUT2, a passive glucose transporter). These proteins were overexpressed in Xenopus oocytes and assayed through volumetric measurements combined with double-electrode electrophysiology or radioactive uptake measurements. The osmotic gradients driving the observed water fluxes were estimated by comparison with the swelling induced by osmotic shocks of known amplitude. We found that, for equivalent cation or glucose uptakes, the combination of substrate accumulations observed with ROMK2 and GLUT2 are sufficient to provide the osmotic gradient necessary to account for a passive water flux through SGLT1. Despite the fact that the Na+/glucose stoichiometry of SGLT1 is 2:1, glucose accumulation accounts for two-thirds of the osmotic gradient responsible for the water flux observed at t = 30 s. It is concluded that the different accumulation processes for neutral versus charged solutes can quantitatively account for the fast water flux associated with Na+/glucose cotransport activation without having to propose the presence of secondary active water transport.
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