ArticleThe Journal of physiology2004
Increase in endothelial cell Ca(2+) in response to mouse cremaster muscle contraction.
Article in The Journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Amplification of endothelium-dependent vasodilatation in contracting human skeletal muscle: role of KThe Journal of physiology · 2019Trial
- Cracking the Endothelial Calcium (CaInternational journal of molecular sciences · 2023Review
- Extracellular matrix fibronectin initiates endothelium-dependent arteriolar dilatation via the heparin-binding, matricryptic RWRPK sequence of the first type III repeat of fibrillar fibronectin.The Journal of physiology · 2016Article
- Local control of blood flow during active hyperaemia: what kinds of integration are important?The Journal of physiology · 2015Review
- Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery.Journal of visualized experiments : JoVE · 2015Article
- Pre-exposure to adenosine, acting via A(2A) receptors on endothelial cells, alters the protein kinase A dependence of adenosine-induced dilation in skeletal muscle resistance arterioles.The Journal of physiology · 2014Article
- Isolation of microvascular endothelial tubes from mouse resistance arteries.Journal of visualized experiments : JoVE · 2013Article
- Low intravascular pressure activates endothelial cell TRPV4 channels, local Ca2+ events, and IKCa channels, reducing arteriolar tone.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Update on vascular endothelial Ca(2+) signalling: A tale of ion channels, pumps and transporters.World journal of biological chemistry · 2012Article
- Chronic hindlimb ischemia impairs functional vasodilation and vascular reactivity in mouse feed arteries.Frontiers in physiology · 2011Article
- Control of muscle blood flow during exercise: local factors and integrative mechanisms.Acta physiologica (Oxford, England) · 2010Review
- Endothelium-dependent vasodilatation and exercise hyperaemia in ageing humans: impact of acute ascorbic acid administration.The Journal of physiology · 2009Article
- Vessel diameter measurement from intravital microscopy.Annals of biomedical engineering · 2009Article
- Enhanced spontaneous Ca2+ events in endothelial cells reflect signalling through myoendothelial gap junctions in pressurized mesenteric arteries.Cell calcium · 2008Article
- Functional vasodilation in the rat spinotrapezius muscle: role of nitric oxide, prostanoids and epoxyeicosatrienoic acids.Clinical and experimental pharmacology & physiology · 2008Article
- Microvascular endothelial cells exhibit optimal aspect ratio for minimizing flow resistance.Annals of biomedical engineering · 2008Article
- A modified fluorescent microsphere-based approach for determining resting and hyperemic blood flows in individual murine skeletal muscles.Vascular pharmacology · 2007Article
- Macromolecule permeability of in situ and excised rodent skeletal muscle arterioles and venules.American journal of physiology. Heart and circulatory physiology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We addressed the role of endothelial cells (ECs) in metabolic dilatation of skeletal muscle arterioles in anaesthetized mice in situ. Electrical field stimulation was used to contract the cremaster muscle for 15 s at 30 Hz. Diameter was observed using bright field microscopy. In controls, muscle contraction produced a 15.7 +/- 1.5 microm dilatation from a baseline of 17.4 +/- 1.6 microm. Endothelial denudation (-EC) via intraluminal perfusion of air abolished this response (1.6 +/- 1.2 microm in -EC, P < 0.05), identifying endothelium as the primary vascular cell type initiating the dilatation. To investigate the role of EC Ca(2+) in metabolic dilatation, arteriolar ECs were loaded with Fluo-4 AM or BAPTA AM by intraluminal perfusion, after which blood flow was re-established. Ca(2+) activity of individual ECs was monitored as a function of change from baseline fluorescence using confocal microscopy. In ECs, whole cell Ca(2+) increased (>10%, P < 0.05) during muscle contraction, and localized Ca(2+) transients were increased (>20%, P < 0.05) during the first minute after contraction. Chelation of EC Ca(2+) abolished the dilatations in response to muscle contraction (1.1 +/- 0.7 microm, P < 0.05). Inhibition of P(1) purinergic receptors (with xanthine amine congener) did not alter the rate of onset of the dilatation (P > 0.05) but decreased its magnitude immediately post stimulation (7.1 +/- 0.9 microm, P < 0.05) and during recovery. These findings demonstrate obligatory roles for endothelium and EC Ca(2+) during metabolic dilatation in intact arterioles. Furthermore, they suggest that at least two separate pathways mediate the local response, one of which involves stimulation of endothelial P(1) purinergic receptors via endogenous adenosine produced during muscle activity.
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