ArticleAntimicrobial agents and chemotherapy2004
Human salivary histatin 5 fungicidal action does not induce programmed cell death pathways in Candida albicans.
Article in Antimicrobial agents and chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Salivary proteins modulate Candida albicans virulence and may prevent oropharingeal candidiasis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Histatin-5 interacts with cellular copper to promote antifungal activity against Candida albicans.Metallomics : integrated biometal science · 2023Article
- Sphingosine as a New Antifungal Agent againstInternational journal of molecular sciences · 2022Article
- Envisaging Antifungal Potential of Histatin 5: A Physiological Salivary Peptide.Journal of fungi (Basel, Switzerland) · 2021Review
- In Vitro and In Vivo Anti-Journal of fungi (Basel, Switzerland) · 2021Article
- Host-Pathogen Interactions Mediated by MDR Transporters in Fungi: As Pleiotropic as it Gets!Genes · 2018Review
- Regulated Cell Death as a Therapeutic Target for Novel Antifungal Peptides and Biologics.Oxidative medicine and cellular longevity · 2018Review
- Sequential and Structural Aspects of Antifungal Peptides from Animals, Bacteria and Fungi Based on Bioinformatics Tools.Probiotics and antimicrobial proteins · 2016Review
- Antifungal Activity and Action Mechanism of Histatin 5-Halocidin Hybrid Peptides against Candida ssp.PloS one · 2016Article
- How does it kill?: understanding the candidacidal mechanism of salivary histatin 5.Eukaryotic cell · 2014Review
- Responses of Candida albicans to the human antimicrobial peptide LL-37.Journal of microbiology (Seoul, Korea) · 2014Article
- Properties and mechanisms of action of naturally occurring antifungal peptides.Cellular and molecular life sciences : CMLS · 2013Review
- Influence of histatin 5 on Candida albicans mitochondrial protein expression assessed by quantitative mass spectrometry.Journal of proteome research · 2011Article
- In vitro reactive oxygen species production by histatins and copper(I,II).Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2009Article
- Histatin 5 initiates osmotic stress response in Candida albicans via activation of the Hog1 mitogen-activated protein kinase pathway.Eukaryotic cell · 2007Article
- Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5.The Biochemical journal · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Salivary histatins (Hsts) are potent candidacidal proteins that induce a nonlytic form of cell death in Candida albicans accompanied by loss of mean cell volume, cell cycle arrest, and elevation of intracellular levels of reactive oxygen species (ROS). Since these phenotypes are often markers of programmed cell death and apoptosis, we investigated whether other classical markers of apoptosis, including generation of intracellular ROS and protein carbonyl groups, chromosomal fragmentation (laddering), and cytochrome c release, are found in Hst 5-mediated cell death. Increased intracellular levels of ROS in C. albicans were detected in cells both following exogenous application of Hst 5 and following intracellular expression of Hst 5. However, Western blot analysis failed to detect specifically increased protein carbonylation in Hst 5-treated cells. There was no evidence of chromosomal laddering and no cytochrome c release was observed following treatment of C. albicans mitochondria with Hst 5. Superoxide dismutase enzymes of C. albicans and Saccharomyces cerevisiae provide essential protection against oxidative stress; therefore, we tested whether SOD mutants have increased susceptibility to Hst 5, as expected if ROS mediate fungicidal effects. Cell survival of S. cerevisiae SOD1/SOD2 mutants and C. albicans SOD1 mutants following Hst 5 treatment (31 micro M) was indistinguishable from the survival of wild-type cells treated with Hst 5. We conclude that ROS may not play a direct role in fungicidal activity and that Hst 5 does not initiate apoptosis or programmed cell death pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.