ArticlePLoS biology2003
Detection and characterization of cellular immune responses using peptide-MHC microarrays.
Article in PLoS biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed.
- Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.PLoS medicine · 2005Trial
- Genetically engineered CD80-pMHC-harboring extracellular vesicles for antigen-specific CD4Frontiers in bioengineering and biotechnology · 2023Article
- Review of the immune mechanisms of preeclampsia and the potential of immune modulating therapy.Human immunology · 2021Review
- Expression and characterization of soluble epitope-defined major histocompatibility complex (MHC) from stable eukaryotic cell lines.Journal of immunological methods · 2019Article
- Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT.Scientific reports · 2017Article
- Evolution of MHC-based technologies used for detection of antigen-responsive T cells.Cancer immunology, immunotherapy : CII · 2017Review
- The functional contribution of calcium ion flux heterogeneity in T cells.Immunology and cell biology · 2015Review
- Review
- Drug-eluting microarrays to identify effective chemotherapeutic combinations targeting patient-derived cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Isolation and cDNA characteristics ofBiotechnology, biotechnological equipment · 2015Article
- Application of the pMHC Array to Characterise Tumour Antigen Specific T Cell Populations in Leukaemia Patients at Disease Diagnosis.PloS one · 2015Article
- Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.PloS one · 2014Article
- The innovative evolution of cancer gene and cellular therapies.Cancer gene therapy · 2013Review
- New targets for the immunotherapy of colon cancer-does reactive disease hold the answer?Cancer gene therapy · 2013Review
- Development of a hypersensitive periodate-cleavable amino acid that is methionine- and disulfide-compatible and its application in MHC exchange reagents for T cell characterisation.Chembiochem : a European journal of chemical biology · 2013Article
- Application of fluorescent monocytes for probing immune complexes on antigen microarrays.PloS one · 2013Article
- Proteomics-based dissection of human endoderm progenitors by differential cell capture on antibody array.Molecular & cellular proteomics : MCP · 2012Article
- Missing: a diagnostic technique to enumerate antigen-specific T cells.Critical reviews in oncology/hematology · 2012Review
- Life on a microarray: assessing live cell functions in a microarray format.Cellular and molecular life sciences : CMLS · 2012Review
- Biosensors for immune cell analysis-A perspective.Biomicrofluidics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection and characterization of antigen-specific T cell populations is critical for understanding the development and physiology of the immune system and its responses in health and disease. We have developed and tested a method that uses arrays of peptide-MHC complexes for the rapid identification, isolation, activation, and characterization of multiple antigen-specific populations of T cells. CD4(+) or CD8(+) lymphocytes can be captured in accordance with their ligand specificity using an array of peptide-MHC complexes printed on a film-coated glass surface. We have characterized the specificity and sensitivity of a peptide-MHC array using labeled lymphocytes from T cell receptor transgenic mice. In addition, we were able to use the array to detect a rare population of antigen-specific T cells following vaccination of a normal mouse. This approach should be useful for epitope discovery, as well as for characterization and analysis of multiple epitope-specific T cell populations during immune responses associated with viral and bacterial infection, cancer, autoimmunity, and vaccination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.