ArticleProceedings of the National Academy of Sciences of the United States of America2004
U4 small nuclear RNA can function in both the major and minor spliceosomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Heterozygosity-Rich Regions in Canine Genome: Can They Serve as Indicators of Balancing Selection?Animals : an open access journal from MDPI · 2025Article
- Taxonomy of introns and the evolution of minor introns.Nucleic acids research · 2024Article
- Clinical interpretation of variants identified in RNU4ATAC, a non-coding spliceosomal gene.PloS one · 2020Article
- U6atac snRNA stem-loop interacts with U12 p65 RNA binding protein and is functionally interchangeable with the U12 apical stem-loop III.Scientific reports · 2016Article
- Splicing diversity revealed by reduced spliceosomes in C. merolae and other organisms.RNA biology · 2015Review
- Functionally important structural elements of U12 snRNA.Nucleic acids research · 2011Article
- The conserved 3' end domain of U6atac snRNA can direct U6 snRNA to the minor spliceosome.RNA (New York, N.Y.) · 2009Article
- Evolution of spliceosomal snRNA genes in metazoan animals.Journal of molecular evolution · 2008Article
- Where in the cell is the minor spliceosome?Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The abundance of the spliceosomal snRNPs is not limiting the splicing of U12-type introns.RNA (New York, N.Y.) · 2006Article
- The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing.Genome biology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
U4 small nuclear RNA (snRNA) and U6 snRNA form a base-paired di-snRNP complex that is essential for pre-mRNA splicing of the major class of metazoan nuclear introns. The functionally analogous but highly diverged U4atac and U6atac snRNAs form a similar complex that is involved in splicing of the minor class of introns. Previous results with mutants of U6atac in which a substructure was replaced by the analogous structure from U6 snRNA suggested that wild-type U4 snRNA might be able to interact productively with the mutant U6atac snRNA. Here we show that a mutant U4 snRNA designed to base pair with a mutant U6atac snRNA can activate U12-dependent splicing when coexpressed in an in vivo genetic suppression assay. This genetic interaction could also be demonstrated in an in vitro crosslinking assay. These results show that a U4/U6atac di-snRNP can correctly splice a U12-dependent intron and suggest that the specificity for spliceosome type resides in the U6 and U6atac small nuclear ribonucleoproteins. Further experiments suggest that expression of a mutant U4 snRNA that can bind to wild-type U6atac snRNA alters the specificity of some splice sites, providing an evolutionary rationale for maintaining two U4-like snRNAs.
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