ArticleInvestigative ophthalmology & visual science2004
Requirement of neuroD for photoreceptor formation in the chick retina.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- New Insights on the Regulatory Gene Network Disturbed in Central Areolar Choroidal Dystrophy-Beyond Classical Gene Candidates.Frontiers in genetics · 2022Pooled it
- Disease-causing mutations in genes encoding transcription factors critical for photoreceptor development.Frontiers in molecular neuroscience · 2023Review
- Retinoid acid and taurine promote NeuroD1-induced differentiation of induced pluripotent stem cells into retinal ganglion cells.Molecular and cellular biochemistry · 2018Article
- The bHLH Transcription Factor NeuroD Governs Photoreceptor Genesis and Regeneration Through Delta-Notch Signaling.Investigative ophthalmology & visual science · 2015Article
- Ophthalmological phenotype associated with homozygous null mutation in the NEUROD1 gene.Molecular vision · 2015Article
- Chick retinal pigment epithelium transdifferentiation assay for proneural activities.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Production of high-titer RCAS retrovirus.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Using the Tg(nrd:egfp)/albino zebrafish line to characterize in vivo expression of neurod.PloS one · 2012Article
- MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1.Nucleic acids research · 2011Article
- Neurog2 controls the leading edge of neurogenesis in the mammalian retina.Developmental biology · 2010Article
- Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors.The Journal of comparative neurology · 2010Article
- Using neurogenin to reprogram chick RPE to produce photoreceptor-like neurons.Investigative ophthalmology & visual science · 2010Article
- Pro-photoreceptor activity of chick neurogenin1.Investigative ophthalmology & visual science · 2009Article
- Neurogenin3 promotes early retinal neurogenesis.Molecular and cellular neurosciences · 2009Article
- Characterization of a transient TCF/LEF-responsive progenitor population in the embryonic mouse retina.Investigative ophthalmology & visual science · 2009Article
- Reprogramming progeny cells of embryonic RPE to produce photoreceptors: development of advanced photoreceptor traits under the induction of neuroD.Investigative ophthalmology & visual science · 2008Article
- Development of the retinotectal system in the direct-developing frog Eleutherodactylus coqui in comparison with other anurans.Frontiers in zoology · 2008Article
- Have we achieved a unified model of photoreceptor cell fate specification in vertebrates?Brain research · 2008Review
- NeuroD1 regulates expression of thyroid hormone receptor 2 and cone opsins in the developing mouse retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Reprogramming chick RPE progeny cells to differentiate towards retinal neurons by ash1.Molecular vision · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe genetic control of photoreceptor cell fate in the vertebrate retina is poorly understood. Published studies suggest that the genetic program underlying photoreceptor production involves neuroD, a proneural basic helix-loop-helix (bHLH) gene. The present study investigates whether neuroD is necessary for photoreceptor cell development, by using loss-of-function analyses.
methodEngrailed-mediated active repression, antisense oligonucleotides, and small interfering RNA (siRNA) were used to attenuate neuroD expression and function in embryonic chick retina. The development of the retina was subsequently analyzed to determine whether these experimental manipulations would yield photoreceptor deficits in otherwise normal retina.
resultsChick embryos infected with retroviruses expressing an active repression construct, En-NeuroDDeltaC, exhibited severe photoreceptor deficits. The outer nuclear layer (ONL) of the retina was no longer a contiguous structure, but became fragmented with regions that contained fewer or no photoreceptor cells. Photoreceptor deficiency was evident even before the retina became laminated, suggesting that active repression of NeuroD may have affected photoreceptor genesis. No deficiency was observed in other types of retinal cells. Culturing retinal cells in the presence of siRNA against neuroD resulted in a more than 50% reduction in the number of photoreceptor cells and an increase in the number of chx10+ cells. Subjecting the developing retina to antisense oligonucleotides against neuroD yielded fewer photoreceptor cells both in vivo and in vitro. Consistent with these observations, anti-NeuroD antibody specifically labeled the nuclei of the ONL.
conclusionsThe data suggest a specific and an essential role of neuroD in photoreceptor formation in the chick retina.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.