ArticleInvestigative ophthalmology & visual science2004
Absence of functional and structural abnormalities associated with expression of EGFP in the retina.
Article in Investigative ophthalmology & visual science, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Validating Fluorescent Chrnb4.EGFP Mouse Models for the Study of Cone Photoreceptor Degeneration.Translational vision science & technology · 2020Article
- A short N-terminal domain of HDAC4 preserves photoreceptors and restores visual function in retinitis pigmentosa.Nature communications · 2015Article
- Characterization of a dominant cone degeneration in a green fluorescent protein-reporter mouse with disruption of Loci associated with human dominant retinal dystrophy.Investigative ophthalmology & visual science · 2011Article
- Evaluation of the adeno-associated virus mediated long-term expression of channelrhodopsin-2 in the mouse retina.Molecular vision · 2009Article
- Barriers for retinal gene therapy: separating fact from fiction.Vision research · 2008Review
- Two-photon microscopy: shedding light on the chemistry of vision.Biochemistry · 2007Review
- Article
- Cone survival despite rod degeneration in XOPS-mCFP transgenic zebrafish.Investigative ophthalmology & visual science · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe present study was undertaken to evaluate the effect of uniform EGFP expression on retinal morphology and function.
methodsElectroretinography (ERG) was used to evaluate the recovery of scotopic a- and b-wave amplitudes after a single 137-cd.sec/m2 flash exposure. The cellular distribution of enhanced green fluorescent protein (EGFP) in the retina and its effect on retinal morphology were evaluated by fluorescence microscopy and histology, respectively. To evaluate its effect on retinal sensitivity to light, EGFP-expressing and control mice were exposed to constant light for 76 hours (3500 lux), and eyes were assessed functionally and structurally at 3 weeks after light exposure.
resultsFluorescence microscopy showed a pronounced EGFP expression in the photoreceptor cell bodies and inner segments. ERG analysis revealed no significant differences in either a- or b-wave amplitudes or recovery between EGFP(+/-) and control mice under dark- or light-adapted conditions. Histologic assessment at as late as 4 months of age showed no difference in retinal morphology or photoreceptor nuclei count in EGFP(+/-) mice when compared with nontransgenic littermates. In addition, evaluation of animals, 3 weeks after constant light exposure, showed no difference between ERG amplitudes, recovery of the scotopic ERG response, or retinal morphology between EGFP(+/-) mice and control animals.
conclusionsFunctional and morphologic evidence shows that long-term, high, uniform levels of EGFP expression have no deleterious effect on the mouse retina. This data demonstrates the safety of EGFP use as an indicator of viral transduction in retinal gene therapy.
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