Evidence map›Paper›PMID 14690679›Full record

ArticleAnalytical biochemistry2004

Radiative decay engineering 3. Surface plasmon-coupled directional emission.

Joseph R Lakowicz

Abstract read
In one paragraph

Article in Analytical biochemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.

0numbers the graph read from it
0cells of the map it votes in
89citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

89 citing papers in PubMed.

  1. Article
  2. Metal-Enhanced Fluorescence for the Biologist's Cellular Imaging Toolkit: Design Principles and Recent Applications.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Fluorophore Coupling to Internal Modes of Bragg Gratings.The journal of physical chemistry. C, Nanomaterials and interfaces · 2020
    Article
  11. Article
  12. Surface plasmon-coupled emission imaging for biological applications.Analytical and bioanalytical chemistry · 2020
    Review
  13. Article
  14. Article
  15. Article
  16. Chemiplasmonics for high-throughput biosensors.International journal of nanomedicine · 2018
    Article
  17. Article
  18. Article
  19. Article
  20. Bloch Surface Wave-Coupled Emission at Ultra-Violet Wavelengths.The journal of physical chemistry. C, Nanomaterials and interfaces · 2016
    Article

29 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Joseph R LakowiczCenter for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA. lakowicz@cfs.umbi.umd.edu

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006
$7.4M
Metallic Surfaces and Particles in DNA AnalysisR01HG002655 · NHGRI · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2003 to 2012
$2.8M
Metal-Enhanced Fluorescence SensingR01EB000682 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2002 to 2006
$1.7M
Biohazard Detection Using Metal-Enhanced FluorescenceR21EB000981 · NIBIB · UNIVERSITY OF MARYLAND BALTIMORE · PI LAKOWICZ, JOSEPH R. · 2002 to 2003
$446k
NCRR NIH HHS P41 RR008119NCRR NIH HHS RR-08119NHGRI NIH HHS HG-002655NHGRI NIH HHS R01 HG002655NIBIB NIH HHS EB-000682NIBIB NIH HHS EB-00981NIBIB NIH HHS R01 EB000682NIBIB NIH HHS R21 EB000981
6 · The paper itself

Abstract

A new method of fluorescence detection that promises to increase sensitivity by 20- to 1000-fold is described. This method will also decrease the contribution of sample autofluorescence to the detected signal. The method depends on the coupling of excited fluorophores with the surface plasmon resonance present in thin metal films, typically silver and gold. The phenomenon of surface plasmon-coupled emission (SPCE) occurs for fluorophores 20-250 nm from the metal surface, allowing detection of fluorophores over substantial distances beyond the metal-sample interface. SPCE depends on interactions of the excited fluorophore with the metal surface. This interaction is independent of the mode of excitation; that is, it does not require evanescent wave or surface-plasmon excitation. In a sense, SPCE is the inverse process of the surface plasmon resonance absorption of thin metal films. Importantly, SPCE occurs over a narrow angular distribution, converting normally isotropic emission into easily collected directional emission. Up to 50% of the emission from unoriented samples can be collected, much larger than typical fluorescence collection efficiencies near 1% or less. SPCE is due only to fluorophores near the metal surface and may be regarded as emission from the induced surface plasmons. Autofluorescence from more distal parts of the sample is decreased due to decreased coupling. SPCE is highly polarized and autofluorescence can be further decreased by collecting only the polarized component or only the light propagating with the appropriate angle. Examples showing how simple optical configurations can be used in diagnostics, sensing, or biotechnology applications are presented. Surface plasmon-coupled emission is likely to find widespread applications throughout the biosciences.

Indexed as

FluorescenceFluorescent DyesModels, TheoreticalSurface Plasmon ResonanceFluorescent Dyes

Identifiers

PMID14690679
PMCPMC2737403

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.