ArticleJournal of protein chemistry2003
Kinetic inactivation study of mushroom tyrosinase: intermediate detection by denaturants.
Article in Journal of protein chemistry, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 22 citations in OpenAlex.
- Denaturation of proteins by SDS and tetraalkylammonium dodecyl sulfates.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Protein unfolding studies of thiol-proteinase inhibitor from goat (Capra hircus) muscle in the presence of urea and GdnHCl as denaturants.European biophysics journal : EBJ · 2011Article
- Differentiation of proteins based on characteristic patterns of association and denaturation in solutions of SDS.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Comparison of guanidine hydrochloride (GdnHCl) and urea denaturation on inactivation and unfolding of human placental cystatin (HPC).The protein journal · 2005Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The unfolding and inhibition study of mushroom tyrosinase have been studied in the presence of different denaturants such as sodium dodecyl sulfate (SDS), guanidine hydrochloride (GdnHCl), and urea. The kinetic two-phase rate constants were commonly measured from semilogarithmic plots of the activity versus time, which resolved into two straight lines, indicating that the inactivation process consisted of fast and slow phases as a first-order reaction. This result also implied that transient partially folded intermediate existed during tyrosinase unfolding pathway. Mushroom tyrosinase had different behaviors to denaturants in regard with: noncooperative binding manner by SDS while cooperative interactions by GdnHCl and urea; in equilibrium state, SDS-micelle never completely inactivated enzyme activity while GdnHCl has single step denaturation and urea induced a typical transition-like process. Various kinetic parameters for each denaturant were calculated and the possible unfolding pathway scheme was discussed.
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