ArticleProceedings of the National Academy of Sciences of the United States of America2004
Hybridoma-free generation of monoclonal antibodies.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Systematic establishment of approaches to the detection of equine macrophage polarization and their application in pathogenic infection.Microbiology spectrum · 2026Article
- Development and application of classical swine fever virus monoclonal antibodies derived from single B cells.Veterinary research · 2023Article
- Epitope-Directed Antibody Elicitation by Genetically Encoded Chemical Cross-Linking Reactivity in the Antigen.ACS central science · 2023Article
- High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay.iScience · 2022Article
- A novel high-throughput single B-cell cloning platform for isolation and characterization of high-affinity and potent SARS-CoV-2 neutralizing antibodies.Antiviral research · 2022Article
- Design and clinical developments of aptamer-drug conjugates for targeted cancer therapy.Biomaterials research · 2021Review
- Advances in Multiplexed Paper-Based Analytical Devices for Cancer Diagnosis: A Review of Technological Developments.Advanced materials technologies · 2021Article
- Direct detection of Strongyloides infection via molecular and antigen detection methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives.International immunopharmacology · 2020Review
- Epitope-directed antibody selection by site-specific photocrosslinking.Science advances · 2020Article
- Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs.Frontiers in microbiology · 2019Article
- Selection of phage-displayed accessible recombinant targeted antibodies (SPARTA): methodology and applications.JCI insight · 2018Article
- Development of monoclonal antibodies against axenic amastigotes ofIranian journal of basic medical sciences · 2018Article
- Construction and immunogenicity of a recombinant swinepox virus expressing a multi-epitope peptide for porcine reproductive and respiratory syndrome virus.Scientific reports · 2017Article
- A High Through-put Platform for Recombinant Antibodies to Folded Proteins.Molecular & cellular proteomics : MCP · 2015Article
- Cloning murine antibody V-genes with non-degenerate primers and conversion to a recombinant antibody format.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2014Article
- A proteomics approach for the identification and cloning of monoclonal antibodies from serum.Nature biotechnology · 2012Article
- Expression profile of human immune-responsive gene 1 and generation and characterization of polyclonal antiserum.Molecular and cellular biochemistry · 2011Article
- Display technologies: application for the discovery of drug and gene delivery agents.Advanced drug delivery reviews · 2006Review
- Phage display mediated immuno-PCR.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Production of monoclonal antibodies requires immortalization of splenocytes by somatic fusion to a myeloma cell line partner (hybridomas). Although hybridomas can be immortal, they may depend on a feeder cell layer and may be genetically unstable. Since the inception of hybridoma technology, efforts to improve efficiency and stability of monoclonal antibody-producing cell lines have not brought about substantial progress. Moreover, suitable human multiple myeloma-derived cell lines for the production of human antibodies have been very difficult to develop. Here we report a strategy that greatly simplifies the generation of antibodies and eliminates the need for hybridomas. We show that splenocytes derived from transgenic mice harboring a mutant temperature-sensitive simian virus 40 large tumor antigen under the control of a mouse major histocompatibility promoter are conditionally immortal at permissive temperatures and produce monoclonal antibodies. This simple approach may become a method of choice for generation and production of both polyclonal and monoclonal antibodies with advantages in high-throughput discovery and antibody-based immunotherapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.