ArticleThe Plant cell2004
KIC, a novel Ca2+ binding protein with one EF-hand motif, interacts with a microtubule motor protein and regulates trichome morphogenesis.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 104 citations in OpenAlex.
- Calcium-Binding Protein and Polymorphism inCurrent issues in molecular biology · 2024Article
- CsTs, a C-type lectin receptor-like kinase, regulates the development trichome development and cuticle metabolism in cucumber (Horticulture research · 2024Article
- Maize and heat stress: Physiological, genetic, and molecular insights.The plant genome · 2024Review
- Transcriptome and hormone metabolome reveal the mechanism of stem bending in water lily (Frontiers in plant science · 2023Article
- A eudicotFrontiers in plant science · 2023Article
- Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.BMC plant biology · 2022Article
- Overexpression of Arabidopsis nucleolar GTP-binding 1 (NOG1) proteins confers drought tolerance in rice.Plant physiology · 2022Article
- Transcriptomic Analysis Reveals LncRNAs Associated with Flowering ofCurrent issues in molecular biology · 2022Article
- Overexpression of GhKTI12 Enhances Seed Yield and Biomass Production in Nicotiana Tabacum.Genes · 2022Article
- Biology of PEST-Containing Nuclear Protein: A Potential Molecular Target for Cancer Research.Frontiers in oncology · 2022Review
- Molecular Mechanisms of Plant Trichome Development.Frontiers in plant science · 2022Review
- Heat Stress-Mediated Constraints in Maize (Frontiers in plant science · 2022Review
- Comparative Transcriptome Analysis of Hard and Tender Fruit Spines of Cucumber to Identify Genes Involved in the Morphological Development of Fruit Spines.Frontiers in plant science · 2022Article
- CmMLO17 and its partner CmKIC potentially support Alternaria alternata growth in Chrysanthemum morifolium.Horticulture research · 2021Article
- Calcium Binding by Arabinogalactan Polysaccharides Is Important for Normal Plant Development.The Plant cell · 2020Article
- Identification, characterization and expression analysis of calmodulin and calmodulin-like proteins in Solanum pennellii.Scientific reports · 2020Article
- CaFrontiers in plant science · 2020Review
- A novel Ca2+-binding protein that can rapidly transduce auxin responses during root growth.PLoS biology · 2019Article
- The Arabidopsis thaliana transcription factor MYB59 regulates calcium signalling during plant growth and stress response.Plant molecular biology · 2019Article
- An Overview of Signaling Regulons During Cold Stress Tolerance in Plants.Current genomics · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinesin-like calmodulin binding protein (KCBP) is a microtubule motor protein involved in the regulation of cell division and trichome morphogenesis. Genetic studies have shown that KCBP is likely to interact with several other proteins. To identify KCBP-interacting proteins, we used the C-terminal region of KCBP in a yeast two-hybrid screen. This screening resulted in the isolation of a novel KCBP-interacting Ca2+ binding protein (KIC). KIC, with its single EF-hand motif, bound Ca2+ at a physiological concentration. Coprecipitation with bacterially expressed protein and native KCBP, gel-mobility shift studies, and ATPase assays with the KCBP motor confirmed that KIC interacts with KCBP in a Ca2+-dependent manner. Interestingly, although both Ca2+-KIC and Ca2+-calmodulin were able to interact with KCBP and inhibit its microtubule binding activity, the concentration of Ca2+ required to inhibit the microtubule-stimulated ATPase activity of KCBP by KIC was threefold less than that required for calmodulin. Two KIC-related Ca2+ binding proteins and a centrin from Arabidopsis, which contain one and four EF-hand motifs, respectively, bound Ca2+ but did not affect microtubule binding and microtubule-stimulated ATPase activities of KCBP, indicating the specificity of Ca2+ sensors in regulating their targets. Overexpression of KIC in Arabidopsis resulted in trichomes with reduced branch number resembling the zwichel/kcbp phenotype. These results suggest that KIC modulates the activity of KCBP in response to changes in cytosolic Ca2+ and regulates trichome morphogenesis.
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