ArticleThe EMBO journal2004
The SNARE Ykt6 mediates protein palmitoylation during an early stage of homotypic vacuole fusion.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 78 citations in OpenAlex.
- Function of the SNARE Ykt6 on autophagosomes requires the Dsl1 complex and the Atg1 kinase complex.EMBO reports · 2020Article
- Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity.Biomolecules · 2020Article
- Ykt6-dependent endosomal recycling is required for Wnt secretion in theDevelopment (Cambridge, England) · 2020Article
- LncRNA PVT1 promotes exosome secretion through YKT6, RAB7, and VAMP3 in pancreatic cancer.Aging · 2020Article
- Article
- Non-canonical role of the SNARE protein Ykt6 in autophagosome-lysosome fusion.PLoS genetics · 2018Article
- Progress toward Understanding Protein S-acylation: Prospective in Plants.Frontiers in plant science · 2017Review
- Vacuolar SNARE protein transmembrane domains serve as nonspecific membrane anchors with unequal roles in lipid mixing.The Journal of biological chemistry · 2015Article
- Distinct palmitoylation events at the amino-terminal conserved cysteines of Env7 direct its stability, localization, and vacuolar fusion regulation in S. cerevisiae.The Journal of biological chemistry · 2014Article
- Article
- Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion.The EMBO journal · 2007Article
- Article
- The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p.The Journal of cell biology · 2005Article
- Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation.The EMBO journal · 2005Article
- Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization.The EMBO journal · 2005Article
- Article
- The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues.The Biochemical journal · 2004Article
- On the mechanism of protein palmitoylation.EMBO reports · 2004Review
- A soluble SNARE drives rapid docking, bypassing ATP and Sec17/18p for vacuole fusion.The EMBO journal · 2004Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NSF homolog Sec18 initiates fusion of yeast vacuoles by disassembling cis-SNARE complexes during priming. Sec18 is also required for palmitoylation of the fusion factor Vac8, although the acylation machinery has not been identified. Here we show that the SNARE Ykt6 mediates Vac8 palmitoylation and acts during a novel subreaction of vacuole fusion. This subreaction is controlled by a Sec17-independent function of Sec18. Our data indicate that Ykt6 presents Pal-CoA via its N-terminal longin domain to Vac8, while transfer to Vac8's SH4 domain occurs spontaneously and not enzymatically. The conservation of Ykt6 and its localization to several organelles suggest that its acyltransferase activity may also be required in other intracellular fusion events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.