ArticleThe EMBO journal2004
What kinesin does at roadblocks: the coordination mechanism for molecular walking.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- An Overview of Contrasting Experimental Results on Dynamics of Kinesin-1 Molecular Motors: Insight into the Underlying Mechanism.Biomolecules · 2025Review
- Kinetic regulation of kinesin's two motor domains coordinates its stepping along microtubules.eLife · 2025Article
- How Kinesin-1 Utilize the Energy of Nucleotide: The Conformational Changes and Mechanochemical Coupling in the Unidirectional Motion of Kinesin-1.International journal of molecular sciences · 2020Review
- These motors were made for walking.Protein science : a publication of the Protein Society · 2020Review
- The ability of the kinesin-2 heterodimer KIF3AC to navigate microtubule networks is provided by the KIF3A motor domain.The Journal of biological chemistry · 2019Article
- Kinesin-2 motors: Kinetics and biophysics.The Journal of biological chemistry · 2018Review
- ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain.Scientific reports · 2017Article
- Kinetic adaptation of human Myo19 for active mitochondrial transport to growing filopodia tips.Scientific reports · 2017Article
- The axonal transport motor kinesin-2 navigates microtubule obstacles via protofilament switching.Traffic (Copenhagen, Denmark) · 2017Article
- Design principles governing chemomechanical coupling of kinesin.Scientific reports · 2017Article
- The structural switch of nucleotide-free kinesin.Scientific reports · 2017Article
- Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.Biophysical reviews · 2015Article
- Kinesin-1 motors can circumvent permanent roadblocks by side-shifting to neighboring protofilaments.Biophysical journal · 2015Article
- Article
- Processivity of the kinesin-2 KIF3A results from rear head gating and not front head gating.The Journal of biological chemistry · 2015Article
- Article
- Stepping and crowding of molecular motors: statistical kinetics from an exclusion process perspective.Biophysical journal · 2014Article
- Multimotor transport in a system of active and inactive kinesin-1 motors.Biophysical journal · 2014Article
- Prime movers: the mechanochemistry of mitotic kinesins.Nature reviews. Molecular cell biology · 2014Review
- Covalent immobilization of microtubules on glass surfaces for molecular motor force measurements and other single-molecule assays.Methods in molecular biology (Clifton, N.J.) · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Competing models for the coordination of processive stepping in kinesin can be tested by introducing a roadblock to prevent lead head attachment. We used T93N, an irreversibly binding mutant monomer, as a roadblock, and measured the rates of nucleotide-induced detachment of kinesin monomers or dimers with and without the T93N roadblock using microflash photolysis combined with stopped flow. Control nucleotide-induced monomer (rK340) unbinding was 73.6 s(-1) for ATP and 40.5 s(-1) for ADP. Control ADP-induced dimer (rK430) unbinding was 18.6 s(-1). Added 20 mM Pi slowed both monomer and dimer unbinding. With the roadblock in place, lead head attachment of dimers is prevented and ATP-induced trail head unbinding was then 42 s(-1). This is less than two-fold slower than the stepping rate of unimpeded rK430 dimers (50-70 s(-1)), indicating that during walking, lead head attachment induces at most only a slight (less than two-fold) acceleration of trail head detachment. As we discuss, this implies a coordination model having very fast (>2000 s(-1)) ATP-induced attachment of the lead head, followed by slower, strain-sensitive ADP release from the lead head.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.