ArticleGut2004
Ferroportin/IREG-1/MTP-1/SLC40A1 modulates the uptake of iron at the apical membrane of enterocytes.
Article in Gut, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- The essential clathrin adapter protein complex-2 is tumor suppressive specifically in vivo.Nature communications · 2025Article
- Iron metabolism and chronic inflammation in IgA nephropathy.Renal failure · 2023Review
- Monocyte MRI Relaxation Rates Are Regulated by Extracellular Iron and Hepcidin.International journal of molecular sciences · 2023Article
- Internalization and Decrease of Duodenal DMT1 Involved in Transient Suppression of Iron Uptake in Short-Acting Mucosal Block.Biological trace element research · 2022Article
- Hepcidin-mediated Iron Regulation in P19 Cells is Detectable by Magnetic Resonance Imaging.Scientific reports · 2020Article
- Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features.Pharmaceuticals (Basel, Switzerland) · 2019Review
- Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.Oncotarget · 2017Article
- Targeting Iron Homeostasis in Acute Kidney Injury.Seminars in nephrology · 2016Review
- Ferroportin deficiency impairs manganese metabolism in flatiron mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015Article
- Iron regulates the expression of ferroportin 1 in the cultured hFOB 1.19 osteoblast cell line.Experimental and therapeutic medicine · 2014Article
- Silencing the Menkes copper-transporting ATPase (Atp7a) gene in rat intestinal epithelial (IEC-6) cells increases iron flux via transcriptional induction of ferroportin 1 (Fpn1).The Journal of nutrition · 2014Article
- Ferroportin and exocytoplasmic ferroxidase activity are required for brain microvascular endothelial cell iron efflux.The Journal of biological chemistry · 2013Article
- A gene expression atlas of the domestic pig.BMC biology · 2012Article
- Profile of altered brain iron acquisition in restless legs syndrome.Brain : a journal of neurology · 2011Article
- Role of L-type Ca2+ channels in iron transport and iron-overload cardiomyopathy.Journal of molecular medicine (Berlin, Germany) · 2006Review
- A role for tumour necrosis factor alpha in human small bowel iron transport.The Biochemical journal · 2005Article
- Augmented internalisation of ferroportin to late endosomes impairs iron uptake by enterocyte-like IEC-6 cells.Pflugers Archiv : European journal of physiology · 2005Article
- Changes in the transcriptional profile of transporters in the intestine along the anterior-posterior and crypt-villus axes.BMC genomics · 2005Article
- Differences in the uptake of iron from Fe(II) ascorbate and Fe(III) citrate by IEC-6 cells and the involvement of ferroportin/IREG-1/MTP-1/SLC40A1.Pflugers Archiv : European journal of physiology · 2004Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAbsorption of non-haeme iron occurs mainly in the duodenum. It involves the divalent metal transporter 1 (DMT1) in the uptake of ferrous Fe(II) iron and the basolateral transporter ferroportin/IREG-1/MTP-1/SLC40A1 in its release. Whether ferroportin functions in this process at other sites in the enterocyte is unknown. In this study the effect of a blocking antibody to ferroportin on the uptake and release of iron was evaluated in enterocyte-like cells (IEC-6 and Caco-2) and in freshly isolated duodenal enterocytes from rats.
methodsUptake of 1 microM Fe(II) and its release by cells was studied in the presence of the antibody. Ferroportin expression was determined by western blot analysis of duodenal mucosa enriched microvillus membranes, Caco-2 cells, IEC-6 cells, and freshly isolated enterocytes. Immunofluorescent detection of ferroporitn was performed on frozen sections of duodenum from rats with variations in body iron stores.
resultsFerroportin was expressed in all cell types. In these cells, the antibody significantly reduced (p<0.05) uptake of Fe(II) by 40-50% but had no effect on the release of iron. In Caco-2 cells, Fe(II) uptake was reduced only when the antibody was in contact with the apical membrane. Ferroportin protein was enriched in microvillus membrane preparations. In enterocytes from iron deficient rats, ferroportin was expressed along the brush border where it colocalised with lactase. Ferroportin was seen in the basal cytoplasm and along the basolateral membranes. Iron loading markedly reduced intracellular expression of ferroportin. In Caco-2 cells, ferroportin also localised to the microvillus and lateral and basal membranes.
conclusionsIn addition to release, ferroportin functions in the uptake of iron at the apical membrane, possibly by modulating the activity of DMT1.
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