ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology1992
Rapid detection of tuberculous and non-tuberculous mycobacteria by polymerase chain reaction amplification of a 162 bp DNA fragment from antigen 85.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Evaluation of the performance of an in-house duplex PCR assay targeting the IS6110 and rpoB genes for tuberculosis diagnosis in Cameroon.BMC infectious diseases · 2020Article
- Article
- Evaluation of the IS6110 PCR assay for the rapid diagnosis of tuberculous meningitis.Cerebrospinal fluid research · 2007Article
- Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction.Journal of clinical microbiology · 2003Article
- Protective efficacy of a DNA vaccine encoding antigen 85A from Mycobacterium bovis BCG against Buruli ulcer.Infection and immunity · 2001Article
- Evaluation of mtp40 genomic fragment amplification for specific detection of Mycobacterium tuberculosis in clinical specimens.Journal of clinical microbiology · 1996Article
- Diagnostic performance of amplified Mycobacterium tuberculosis direct test with cerebrospinal fluid, other nonrespiratory, and respiratory specimens.Journal of clinical microbiology · 1996Article
- Contribution of the polymerase chain reaction to the diagnosis of tuberculous infections in children.European journal of pediatrics · 1996Article
- Multiprimer PCR system for differential identification of mycobacteria in clinical samples.Journal of clinical microbiology · 1996Article
- Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.Journal of clinical microbiology · 1995Article
- Rapid detection of tuberculous and non-tuberculous mycobacteria by microscopic observation of growth on Middlebrook 7H11 agar.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1995Article
- The new diagnostic mycobacteriology laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1994Review
- Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system.Journal of clinical microbiology · 1994Article
- Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 1993Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A polymerase chain reaction (PCR) assay was developed for detection of mycobacteria using amplification of a 162 bp region of the genes coding for the mycobacterial antigen 85 complex. Strains belonging to the Mycobacterium tuberculosis complex were further differentiated from non-tuberculous mycobacteria by hybridization of the PCR derived Southern blot with an internal oligonucleotide probe and washing under stringent conditions. The method allowed rapid and sensitive detection of mycobacterial DNA in uncultured clinical samples. PCR results obtained for Mycobacterium tuberculosis in 206 specimens from 180 untreated patients gave a sensitivity of 93.9% and a specificity of 94.3% compared with the culture. PCR detected DNA from Mycobacterium tuberculosis in seven samples from patients with clinically evident tuberculosis in whom culture was negative. The results suggest that this PCR assay could be used for early and specific diagnosis of tuberculosis.
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