ArticlePlant molecular biology2003
Auxin distribution in Lotus japonicus during root nodule development.
Article in Plant molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 132 citations in OpenAlex.
- Systemic proteome remodeling in soybean during early nodulation: distinct responses in roots and leaves.Frontiers in plant science · 2026Article
- Nodule organogenesis in Medicago truncatula requires local stage-specific auxin biosynthesis and transport.Plant physiology · 2025Article
- Genome-wide analysis of PIN genes in cultivated peanuts (Arachis hypogaea L.): identification, subcellular localization, evolution, and expression patterns.BMC genomics · 2023Article
- Exogenously Applied Cytokinin Altered the Bacterial Release and Subsequent Stages of Nodule Development in PeaPlants (Basel, Switzerland) · 2023Article
- On the trail of auxin: Reporters and sensors.The Plant cell · 2022Review
- Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event inInternational journal of molecular sciences · 2022Article
- Roles of non-coding RNAs in the hormonal and nutritional regulation in nodulation and nitrogen fixation.Frontiers in plant science · 2022Review
- Multimodal Spectroscopic Imaging of Pea Root Nodules to Assess the Nitrogen Fixation in the Presence of Biofertilizer Based on Nod-Factors.International journal of molecular sciences · 2021Article
- GmPIN-dependent polar auxin transport is involved in soybean nodule development.The Plant cell · 2021Article
- TheInternational journal of molecular sciences · 2021Article
- Lotus japonicus Nuclear Factor YA1, a nodule emergence stage-specific regulator of auxin signalling.The New phytologist · 2021Article
- No Home without Hormones: How Plant Hormones Control Legume Nodule Organogenesis.Plant communications · 2020Review
- The impact of the rhizobia-legume symbiosis on host root system architecture.Journal of experimental botany · 2020Review
- Article
- NODULE INCEPTION Recruits the Lateral Root Developmental Program for Symbiotic Nodule Organogenesis in Medicago truncatula.Current biology : CB · 2019Article
- GmYUC2a mediates auxin biosynthesis during root development and nodulation in soybean.Journal of experimental botany · 2019Article
- A convenient, soil-free method for the production of root nodules in soybean to study the effects of exogenous additives.Plant direct · 2019Article
- Expression Analysis ofInternational journal of molecular sciences · 2019Article
- A Toolkit for High Resolution Imaging of Cell Division and Phytohormone Signaling in Legume Roots and Root Nodules.Frontiers in plant science · 2019Article
- Filling the Gap: Functional Clustering of ABC Proteins for the Investigation of Hormonal TransportFrontiers in plant science · 2019Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For this work, Lotus japonicus transgenic plants were constructed expressing a fusion reporter gene consisting of the genes beta-glucuronidase (gus) and green fluorescent protein (gfp) under control of the soybean auxin-responsive promoter GH3. These plants expressed GUS and GFP in the vascular bundle of shoots, roots and leafs. Root sections showed that in mature parts of the roots GUS is mainly expressed in phloem and vascular parenchyma of the vascular cylinder. By detecting GUS activity, we describe the auxin distribution pattern in the root of the determinate nodulating legume L. japonicus during the development of nodulation and also after inoculation with purified Nod factors, N-naphthylphthalamic acid (NPA) and indoleacetic acid (IAA). Differently than white clover, which forms indeterminate nodules, L. japonicus presented a strong GUS activity at the dividing outer cortical cells during the first nodule cell divisions. This suggests different auxin distribution pattern between the determinate and indeterminate nodulating legumes that may be responsible of the differences in nodule development between these groups. By measuring of the GFP fluorescence expressed 21 days after treatment with Nod factors or bacteria we were able to quantify the differences in GH3 expression levels in single living roots. In order to correlate these data with auxin transport capacity we measured the auxin transport levels by a previously described radioactive method. At 48 h after inoculation with Nod factors, auxin transport showed to be increased in the middle root segment. The results obtained indicate that L. japonicus transformed lines expressing the GFP and GUS reporters under the control of the GH3 promoter are suitable for the study of auxin distribution in this legume.
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