ArticlePlant physiology2003
Light control of nuclear gene mRNA abundance and translation in tobacco.
Article in Plant physiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- From the archives: oxylipins, trojan horses, and light-dependent mRNA stabilization.The Plant cell · 2023Article
- N6-methyladenosine mRNA methylation is important for the light response in soybean.Frontiers in plant science · 2023Article
- Light Activates the Translational Regulatory Kinase GCN2 via Reactive Oxygen Species Emanating from the Chloroplast.The Plant cell · 2020Article
- Light-Dependent Activation of the GCN2 Kinase Under Cold and Salt Stress Is Mediated by the Photosynthetic Status of the Chloroplast.Frontiers in plant science · 2020Article
- Recent Discoveries on the Role of TOR (Target of Rapamycin) Signaling in Translation in Plants.Plant physiology · 2018Review
- Phosphorylation of Ribosomal Protein RPS6 Integrates Light Signals and Circadian Clock Signals.Frontiers in plant science · 2017Article
- Unique features of the m6A methylome in Arabidopsis thaliana.Nature communications · 2014Article
- Proteins with high turnover rate in barley leaves estimated by proteome analysis combined with in planta isotope labeling.Plant physiology · 2014Article
- Post-transcriptional control of light-harvesting genes expression under light stress.Plant molecular biology · 2013Article
- Dynamic Light Regulation of Translation Status in Arabidopsis thaliana.Frontiers in plant science · 2012Article
- XAP5 CIRCADIAN TIMEKEEPER coordinates light signals for proper timing of photomorphogenesis and the circadian clock in Arabidopsis.The Plant cell · 2008Article
- Plastid cues posttranscriptionally regulate the accumulation of key enzymes of the methylerythritol phosphate pathway in Arabidopsis.Plant physiology · 2006Article
- Article
- The early dark-response in Arabidopsis thaliana revealed by cDNA microarray analysis.Plant molecular biology · 2006Article
- NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii.The Plant cell · 2005Article
- The Fed-1 (CAUU)4 element is a 5' UTR dark-responsive mRNA instability element that functions independently of dark-induced polyribosome dissociation.Plant molecular biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photosynthetic signals modulate expression of nuclear genes at the levels of mRNA transcription, mRNA stability, and translation. In transgenic tobacco (Nicotiana tabacum), the pea (Pisum sativum) Ferredoxin 1 (Fed-1) mRNA dissociates from polyribosomes and becomes destabilized when photosynthesis is inhibited by photosynthetic electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea. We used polymerase chain reaction suppressive-subtractive hybridization to identify similarly regulated endogenous tobacco genes. This screen identified 14 nuclear-encoded tobacco mRNAs whose light-induced increase in abundance is suppressed in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Sequence analysis of the cognate cDNAs revealed that nine of the mRNAs encode putative chloroplast-targeted proteins. We asked whether the abundance of these mRNAs was regulated transcriptionally or posttranscriptionally. Of the five mRNAs with sufficient abundance to detect using nuclear run-on assays, we observed transcriptional regulation of alpha-tubulin, thiazole biosynthetic enzyme, and pSKA10 (an unknown gene). Photosystem A subunit L and, to a lesser extent, alpha-tubulin and pSKA10 mRNAs, may also be stabilized in the light. In contrast, Rubisco small subunit mRNA abundance appears to be transcriptionally up-regulated but posttranscriptionally down-regulated in the light. To determine whether, like Fed-1 mRNA, the mRNAs identified in this screen were translationally responsive to light, we characterized the polyribosome association of these mRNAs in the light and after a 15-min dark treatment. A subset of the mRNAs showed dramatic dark-induced polyribosome dissociation, similar to Fed-1 mRNA, and all of the mRNAs showed at least slight polyribosome dissociation. Thus, both posttranscriptional and translational regulation appear to be important mechanisms regulating the expression of many nuclear-encoded mRNAs encoding proteins involved in photosynthesis.
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Registered trials
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