ArticleCell communication & adhesion
Pore-lining residues identified by single channel SCAM studies in Cx46 hemichannels.
Article in Cell communication & adhesion. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Association of variants in GJA8 with familial acorea-microphthalmia-cataract syndrome.European journal of human genetics : EJHG · 2024Article
- Connexin 46 and connexin 50 gap junction channel properties are shaped by structural and dynamic features of their N-terminal domains.The Journal of physiology · 2021Article
- Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å.Nature communications · 2020Article
- Article
- LRRC8 N termini influence pore properties and gating of volume-regulated anion channels (VRACs).The Journal of biological chemistry · 2018Article
- The NHAmerican journal of physiology. Cell physiology · 2017Article
- Stochastic Model of Gap Junctions Exhibiting Rectification and Multiple Closed States of Slow Gates.Biophysical journal · 2016Article
- Reverberation of excitation in neuronal networks interconnected through voltage-gated gap junction channels.The Journal of general physiology · 2016Article
- Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules.The Journal of general physiology · 2015Article
- Aberrant connexin26 hemichannels underlying keratitis-ichthyosis-deafness syndrome are potently inhibited by mefloquine.The Journal of investigative dermatology · 2015Article
- Functional effects of Cx50 mutations associated with congenital cataracts.American journal of physiology. Cell physiology · 2014Article
- Connexin channel modulators and their mechanisms of action.Neuropharmacology · 2013Review
- Neurological manifestations of oculodentodigital dysplasia: a Cx43 channelopathy of the central nervous system?Frontiers in pharmacology · 2013Review
- The N-terminal half of the connexin protein contains the core elements of the pore and voltage gates.The Journal of membrane biology · 2012Article
- Mechanism of inhibition of connexin channels by the quinine derivative N-benzylquininium.The Journal of general physiology · 2012Article
- Differentially altered Ca2+ regulation and Ca2+ permeability in Cx26 hemichannels formed by the A40V and G45E mutations that cause keratitis ichthyosis deafness syndrome.The Journal of general physiology · 2010Article
- Loop gating of connexin hemichannels involves movement of pore-lining residues in the first extracellular loop domain.The Journal of biological chemistry · 2009Article
- Divalent cations regulate connexin hemichannels by modulating intrinsic voltage-dependent gating.The Journal of general physiology · 2008Article
- A fully atomistic model of the Cx32 connexon.PloS one · 2008Article
- Site-directed mutagenesis reveals putative regions of protein interaction within the transmembrane domains of connexins.Cell communication & adhesion · 2008Article
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Authors and funding
5 authors.
Funding
Abstract
The substituted cysteine accessibility method was applied to single Cx46 hemichannels to identify residues that participate in lining the aqueous pore of channels formed of connexins. Criteria for assignment to the pore included reactivity to sulfydryl-specific methanethiosulfonate (MTS) reagents from both sides of an open hemichannel and observable effects on open channel properties. We demonstrate reactivity to MTS reagents over a stretch of seventeen amino acids, D51 through L35, that constitute segments of E1 and TM1. Qualitatively, the nature of the effects caused by the Cys substitutions alone and their modification with MTS reagents of either charge indicate side chain valence is most influential in determining single channel properties with D51 and L35 defining the extracellular and intracellular limits, respectively, of the identified pore-lining region. A number of Cys substitutions beyond L35 in TM1 caused severe alterations in hemichannel function and precluded assignment to the pore. Although all six subunits can be modified by smaller MTS reagents, modifications appear limited to fewer subunits with larger reagents.
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