ArticleArthritis research & therapy2003
Quantitative biomarker analysis of synovial gene expression by real-time PCR.
Article in Arthritis research & therapy, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it, 132 citations in OpenAlex.
- Pooled it
- Toreforant, an orally active histamine HInflammation research : official journal of the European Histamine Research Society ... [et al.] · 2019Trial
- The JAK inhibitor tofacitinib suppresses synovial JAK1-STAT signalling in rheumatoid arthritis.Annals of the rheumatic diseases · 2015Trial
- A prospective, randomised, placebo-controlled study to identify biomarkers associated with active treatment in psoriatic arthritis: effects of adalimumab treatment on synovial tissue.Annals of the rheumatic diseases · 2009Trial
- Synovial tissue response to rituximab: mechanism of action and identification of biomarkers of response.Annals of the rheumatic diseases · 2008Trial
- Assessment of rituximab's immunomodulatory synovial effects (ARISE trial). 1: clinical and synovial biomarker results.Annals of the rheumatic diseases · 2008Trial
- Real-time quantitative PCR to detect changes in synovial gene expression in rheumatoid arthritis after corticosteroid treatment.Annals of the rheumatic diseases · 2007Trial
- Pretreatment macrophage infiltration of the synovium predicts the clinical effect of both radiation synovectomy and intra-articular glucocorticoids.Annals of the rheumatic diseases · 2006Trial
- Analytical Quality by Design-Based Reverse-Phase High-Performance Liquid Chromatography Method Development for Biomarker: A Comprehensive Review of Quality Assessment and Analytical Trends.Biomedical chromatography : BMC · 2026Review
- Determination of the most suitable reference gene for transcription analysis of synovial tissue from osteoarthritis patients.Scientific reports · 2026Article
- Joint-specific regulation of homeobox D10 expression in rheumatoid arthritis fibroblast-like synoviocytes.PloS one · 2024Article
- The histopathological synovitis score is influenced by biopsy location in patients with knee osteoarthritis.Archives of orthopaedic and trauma surgery · 2022Article
- Improving Transcriptome Fidelity Following Synovial Tissue Disaggregation.Frontiers in medicine · 2022Article
- Susceptibility of cyclin-dependent kinase inhibitor 1-deficient mice to rheumatoid arthritis arising from interleukin-1β-induced inflammation.Scientific reports · 2021Article
- Editorial: Synovial Tissue: Turning the Page to Precision Medicine in Arthritis?Frontiers in medicine · 2021Article
- Joint Location-Specific JAK-STAT Signaling in Rheumatoid Arthritis Fibroblast-like Synoviocytes.ACR open rheumatology · 2019Article
- Cartilage-binding antibodies induce pain through immune complex-mediated activation of neurons.The Journal of experimental medicine · 2019Article
- Synovial Tissue Biopsy Collection by Rheumatologists: Ready for Clinical Implementation?Frontiers in medicine · 2019Review
- Exploring the transcriptome of resident spinal microglia after collagen antibody-induced arthritis.Pain · 2019Article
- Clinical Applications of Synovial Biopsy.Frontiers in medicine · 2019Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synovial biomarker analysis in rheumatoid arthritis can be used to evaluate drug effect in clinical trials of novel therapeutic agents. Previous studies of synovial gene expression for these studies have mainly relied on histological methods including immunohistochemistry and in situ hybridization. To increase the reliability of mRNA measurements on small synovial tissue samples, we developed and validated real time quantitative PCR (Q-PCR) methods on biopsy specimens. RNA was isolated from synovial tissue and cDNA was prepared. Cell-based standards were prepared from mitogen-stimulated peripheral blood mononuclear cells. Real time PCR was performed using TaqMan chemistry to quantify gene expression relative to the cell-based standard. Application of the cellular standard curve method markedly reduced intra- and inter-assay variability and corrected amplification efficiency errors compared with the C(t) method. The inter-assay coefficient of variation was less than 25% over time. Q-PCR methods were validated by demonstrating increased expression of IL-1beta and IL-6 expression in rheumatoid arthritis synovial samples compared with osteoarthritis synovium. Based on determinations of sampling error and coefficient of variation, twofold differences in gene expression in serial biopsies can be detected by assaying approximately six synovial tissue biopsies from 8 to 10 patients. These data indicate that Q-PCR is a reliable method for determining relative gene expression in small synovial tissue specimens. The technique can potentially be used in serial biopsy studies to provide insights into mechanism of action and therapeutic effect of new anti-inflammatory agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.