ArticleJournal of bacteriology2004
Fine-tuning in regulation of Clp protein content in Bacillus subtilis.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 90 citations in OpenAlex.
- Proteotoxic Stress Bioreporter Enables Mechanism-Informed Antibiotic Discovery.Journal of natural products · 2026Article
- Repurposing Birabresib to target Gram‑positive bacteria.npj antimicrobials and resistance · 2026Article
- Structure-function analysis of the bacterial ClpE-ClpP AAA+ protease.The Journal of biological chemistry · 2026Article
- Unraveling proteomic chaos by independent component analysis-ClpX proficiency promotes the iron and oxygen limitation responses ofmSystems · 2025Article
- Characterization of the MgsR-dependent promoter structure in Bacillus subtilis-application of a novel pHIS plasmid-based screening system for promoter element analysis.Nucleic acids research · 2025Article
- pTripleTREP - A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus.Microbial cell factories · 2025Article
- ClpP2 proteasomes and SpxA1 determine Listeria monocytogenes tartrolon B hyper-resistance.PLoS genetics · 2025Article
- Requirement of ClpX for CtsR dissociation from its operator elements upon heat stress inFrontiers in microbiology · 2025Article
- Activation of the general stress response sigma factor SigB prevents competence development inmBio · 2024Article
- Diverse nature of ClpX degradation motifs inMicrobiology spectrum · 2024Article
- Whole genome sequence analyses of thermotolerant Bacillus sp. isolates from food.Genomics & informatics · 2023Article
- (p)ppGpp - an important player during heat shock response.microLife · 2023Review
- Clp ATPases differentially affect natural competence development in Streptococcus mutans.MicrobiologyOpen · 2022Article
- MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling.PLoS pathogens · 2022Article
- Cellular functions of the ClpP protease impacting bacterial virulence.Frontiers in molecular biosciences · 2022Review
- Resisting the Heat: Bacterial Disaggregases Rescue Cells From Devastating Protein Aggregation.Frontiers in molecular biosciences · 2021Review
- Mitochondrial ClpP serine protease-biological function and emerging target for cancer therapy.Cell death & disease · 2020Review
- Comparative Genome Analysis ofMicroorganisms · 2020Article
- Induction ofMicrobiology (Reading, England) · 2020Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clp-controlled proteolysis in Bacillus subtilis seems to play a substantial role, particularly under stress conditions. Calibrated Western blot analyses were used to estimate the approximate numbers of heat-inducible Clp molecules within a single cell. According to these numbers, the different Clp ATPases do not seem to compete for the proteolytic subunit ClpP. Coimmunoprecipitation experiments revealed the predicted specific ClpX-ClpP, ClpC-ClpP, and ClpE-ClpP interactions. ClpE and ClpX are rapidly degraded in wild-type cells during permanent heat stress but remained almost stable in a clpP mutant, suggesting ClpP-dependent degradation. In particular, ClpCP appeared to be involved in the degradation of the short-lived ClpE ATPase, indicating a negative "autoregulatory" circuit for this particular Clp ATPase at the posttranslational level. Analysis of the half-life of stress-inducible clp mRNAs during exponential growth and heat shock revealed precise regulation of the synthesis of each Clp protein at the posttranscriptional level as well to meet the needs of B. subtilis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.