ArticleJournal of bacteriology2004
Growth phase-dependent regulation and stringent control of fis are conserved processes in enteric bacteria and involve a single promoter (fis P) in Escherichia coli.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Fis suppresses late-stage virulence gene expression in Yersinia pseudotuberculosis at environmental temperatures.PLoS pathogens · 2026Article
- The Nucleoid-Associated Protein Fis Represses Type 3 Fimbriae to Modulate Biofilm and Adherence Formation inMicroorganisms · 2025Article
- Inactivation ofMicrobiology spectrum · 2023Article
- The Biological and Regulatory Role of Type VI Secretion System ofInfection and drug resistance · 2023Review
- Genome-Wide Investigation of Pasteurella multocida Identifies the Stringent Response as a Negative Regulator of Hyaluronic Acid Capsule Production.Microbiology spectrum · 2022Article
- The Fis Nucleoid Protein Negatively Regulates the Phase VariationFrontiers in microbiology · 2022Article
- Fis Connects Two Sensory Pathways, Quorum Sensing and Surface Sensing, to Control Motility inFrontiers in microbiology · 2021Article
- Insights into Klebsiella pneumoniae type VI secretion system transcriptional regulation.BMC genomics · 2019Article
- Endogenous and Foreign Nucleoid-Associated Proteins of Bacteria: Occurrence, Interactions and Effects on Mobile Genetic Elements and Host's Biology.Computational and structural biotechnology journal · 2019Review
- Fis Regulates Type III Secretion System by Influencing the Transcription ofFrontiers in microbiology · 2017Article
- The physiology of growth arrest: uniting molecular and environmental microbiology.Nature reviews. Microbiology · 2016Review
- An Interplay among FIS, H-NS, and Guanosine Tetraphosphate Modulates Transcription of the Escherichia coli cspA Gene under Physiological Growth Conditions.Frontiers in molecular biosciences · 2016Article
- Dynamic Transcriptional Regulation of Fis in Salmonella During the Exponential Phase.Current microbiology · 2015Article
- Upstream binding of idling RNA polymerase modulates transcription initiation from a nearby promoter.The Journal of biological chemistry · 2015Article
- Regulation Systems of Bacteria such as Escherichia coli in Response to Nutrient Limitation and Environmental Stresses.Metabolites · 2013Article
- Shared control of gene expression in bacteria by transcription factors and global physiology of the cell.Molecular systems biology · 2013Article
- Transmission of an oxygen availability signal at the Salmonella enterica serovar Typhimurium fis promoter.PloS one · 2013Article
- Robust translation of the nucleoid protein Fis requires a remote upstream AU element and is enhanced by RNA secondary structure.Journal of bacteriology · 2012Article
- Partially observed bipartite network analysis to identify predictive connections in transcriptional regulatory networks.BMC systems biology · 2011Article
- The role of the local environment of engineered Tyr to Trp substitutions for probing the denaturation mechanism of FIS.Protein science : a publication of the Protein Society · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The intracellular concentration of the Escherichia coli factor for inversion stimulation (Fis), a global regulator of transcription and a facilitator of certain site-specific DNA recombination events, varies substantially in response to changes in the nutritional environment and growth phase. Under conditions of nutritional upshift, fis is transiently expressed at very high levels, whereas under induced starvation conditions, fis is repressed by stringent control. We show that both of these regulatory processes operate on the chromosomal fis genes of the enterobacteria Klebsiella pneumoniae, Serratia marcescens, Erwinia carotovora, and Proteus vulgaris, strongly suggesting that the physiological role of Fis is closely tied to its transcriptional regulation in response to the nutritional environment. These transcriptional regulatory processes were previously shown to involve a single promoter (fis P) preceding the fis operon in E. coli. Recent work challenged this notion by presenting evidence from primer extension assays which appeared to indicate that there are multiple promoters upstream of fis P that contribute significantly to the expression and regulation of fis in E. coli. Thus, a rigorous analysis of the fis promoter region was conducted to assess the contribution of such additional promoters. However, our data from primer extension analysis, S1 nuclease mapping, beta-galactosidase assays, and in vitro transcription analysis all indicate that fis P is the sole E. coli fis promoter in vivo and in vitro. We further show how certain conditions used in the primer extension reactions can generate artifacts resulting from secondary annealing events that are the likely source of incorrect assignment of additional fis promoters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.