ArticleJournal of bacteriology2004
Temporal expression of pertussis toxin and Ptl secretion proteins by Bordetella pertussis.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Mechanisms of Pertussis Toxin Action: ADP-Ribosylation and Its Role in Pertussis Pathogenesis.Toxins · 2026Review
- A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities.Frontiers in microbiology · 2022Article
- Diversity of secretion systems associated with virulence characteristics of the classical bordetellae.Microbiology (Reading, England) · 2015Article
- Identification and characterization of the carbohydrate ligands recognized by pertussis toxin via a glycan microarray and surface plasmon resonance.Biochemistry · 2010Article
- Mycoplasma pneumoniae Community Acquired Respiratory Distress Syndrome toxin expression reveals growth phase and infection-dependent regulation.Molecular microbiology · 2010Article
- Synergic effect of genotype changes in pertussis toxin and pertactin on adaptation to an acellular pertussis vaccine in the murine intranasal challenge model.Clinical and vaccine immunology : CVI · 2010Article
- Characterization of a unique ADP-ribosyltransferase of Mycoplasma penetrans.Infection and immunity · 2009Article
- Pertussis toxin signals through the TCR to initiate cross-desensitization of the chemokine receptor CXCR4.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Virulence factor secretion and translocation by Bordetella species.Current opinion in microbiology · 2009Review
- Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence.Cellular microbiology · 2008Review
- Pertussis toxin utilizes proximal components of the T-cell receptor complex to initiate signal transduction events in T cells.Infection and immunity · 2007Article
- Growth phase- and nutrient limitation-associated transcript abundance regulation in Bordetella pertussis.Infection and immunity · 2006Article
- Comparison of the genome sequence of the poultry pathogen Bordetella avium with those of B. bronchiseptica, B. pertussis, and B. parapertussis reveals extensive diversity in surface structures associated with host interaction.Journal of bacteriology · 2006Article
- Analysis of subassemblies of pertussis toxin subunits in vivo and their interaction with the ptl transport apparatus.Infection and immunity · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Pertussis toxin is an AB(5) toxin comprised of protein subunits S1 through S5. The individual subunits are secreted by a Sec-dependent mechanism into the periplasm, where the toxin is assembled. The Ptl type IV secretion system mediates secretion of assembled toxin past the outer membrane. In this study, we examined the time course of protein expression, toxin assembly, and secretion as a function of the bacterial growth cycle. Logarithmic growth was observed after a 1-h lag phase. Secreted toxin was first observed at 3 h. Secretion continued throughout the logarithmic growth phase and decreased as the culture entered the stationary phase after about 24 h. On a per cell basis, toxin secretion occurred at a constant rate of 3 molecules/min/cell from 2 to 18 h. More of toxin subunits S1, S2, and S3 were produced than were secreted, resulting in periplasmic accumulation. Periplasmic S1, S2, and S3 were found to be soluble in the periplasm, as well as membrane associated. About one-half of the periplasmic S1, S2 and S3 subunits were incorporated into holotoxin. Secretion component PtlF was present at a low level at time zero, and the level increased between 2 and 24 h from 30 to 1,000 molecules per cell; however, the initial level of PtlF, 30 molecules per cell, supported maximal secretion. The accumulation of both periplasmic toxin and secretion components suggests that translation rates exceed the rate of secretion and that secretion, not toxin and Ptl complex assembly, is rate limiting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.