ArticleProceedings of the National Academy of Sciences of the United States of America2003
Genetic, biochemical, and morphological evidence for the involvement of N-glycosylation in biosynthesis of the cell wall beta1,6-glucan of Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Abrogation of glucosidase I-mediated glycoprotein deglucosylation results in a sick phenotype in fission yeasts: Model for the human MOGS-CDG disorder.The Journal of biological chemistry · 2018Article
- CaGdt1 plays a compensatory role for the calcium pump CaPmr1 in the regulation of calcium signaling and cell wall integrity signaling in Candida albicans.Cell communication and signaling : CCS · 2018Article
- The oligosaccharyl transferase subunit STT3 mediates fungal development and is required for virulence in Verticillium dahliae.Current genetics · 2018Article
- Hypoxia-elicited impairment of cell wall integrity, glycosylation precursor synthesis, and growth in scaled-up high-cell density fed-batch cultures of Saccharomyces cerevisiae.Microbial cell factories · 2016Article
- Article
- MrSkn7 controls sporulation, cell wall integrity, autolysis, and virulence in Metarhizium robertsii.Eukaryotic cell · 2015Article
- The Fusarium oxysporum gnt2, encoding a putative N-acetylglucosamine transferase, is involved in cell wall architecture and virulence.PloS one · 2013Article
- Review
- Candida albicans cell wall proteins.Microbiology and molecular biology reviews : MMBR · 2008Review
- Influence of N-glycosylation on Saccharomyces cerevisiae morphology: a golgi glycosylation mutant shows cell division defects.Current microbiology · 2007Article
- Genome-wide analysis of the response to protein glycosylation deficiency in yeast.FEMS yeast research · 2006Article
- Cell wall assembly in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006Review
- KRE5 gene null mutant strains of Candida albicans are avirulent and have altered cell wall composition and hypha formation properties.Eukaryotic cell · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Recent evidence indicates that Stt3p plays a central role in the recognition and/or catalytic step in N-glycosylation (asparagine-linked glycosylation) in the lumen of the endoplasmic reticulum. It is known that stt3 mutants exhibit certain phenotypic features that are suggestive of a cell wall defect. To understand the basis of these phenotypes, we devised a genetic screen to isolate strains bearing mutations that lead to synthetic lethality in combination with the stt3-1 mutation. Using this screen, we were surprised to identify two KRE genes (KRE5 and KRE9) that are involved in the biosynthesis of the cell wall beta1,6-glucan. This finding led us to propose that the N-glycosylation process is essential in the biosynthesis of cell wall beta1,6-glucan. This proposal was supported by the observation that several stt3 mutants exhibited a 60-70% reduction in the content of cell wall beta1,6-glucan as compared with WT cells. Transmission electron microscopy revealed that the stt3 mutant strains exhibit a diffused cell wall with loss of the outer mannoprotein layer as compared with the WT cells. Thus, we provide genetic, morphological, and biochemical evidence for the critical involvement of N-glycosylation in some step in assembly of the cell wall beta1,6-glucan in Saccharomyces cerevisiae.
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