ArticleBMC genomics2003
Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis.
Article in BMC genomics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 74 citations in OpenAlex.
- Comparative Genomics of Sigma Factors inMicroorganisms · 2025Article
- Identification of virulence genes and clade-specific markers through pan-genomic analysis of Leptospira.BMC microbiology · 2025Article
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- Genome features of a novel hydrocarbonoclastic Chryseobacterium oranimense strain and its comparison to bacterial oil-degraders and to other C. oranimense strains.DNA research : an international journal for rapid publication of reports on genes and genomes · 2023Article
- Transcriptional Responses ofFrontiers in microbiology · 2021Article
- Depth-related variability in viral communities in highly stratified sulfidic mine tailings.Microbiome · 2020Article
- Whole genome sequence analysis reveals high genetic variation of newly isolated Acidithiobacillus ferrooxidans IO-2C.Scientific reports · 2019Article
- Integration of Biological Networks forFrontiers in molecular biosciences · 2019Article
- Microbial Communities Associated With Passive Acidic Abandoned Coal Mine Remediation.Frontiers in microbiology · 2019Article
- Acidithiobacillus ferrooxidans and its potential application.Extremophiles : life under extreme conditions · 2018Review
- Microbial community and metabolic pathway succession driven by changed nutrient inputs in tailings: effects of different nutrients on tailing remediation.Scientific reports · 2017Article
- Putative bacterial interactions from metagenomic knowledge with an integrative systems ecology approach.MicrobiologyOpen · 2016Article
- Article
- The sulfur/sulfonates transport systems in Xanthomonas citri pv. citri.BMC genomics · 2015Article
- Differential expression of sulfur assimilation pathway genes in Acidithiobacillus ferrooxidans under Cd²⁺ stress: evidence from transcriptional, enzymatic, and metabolic profiles.Extremophiles : life under extreme conditions · 2015Article
- Microbial diversity and metabolic networks in acid mine drainage habitats.Frontiers in microbiology · 2015Review
- Anaerobic sulfur metabolism coupled to dissimilatory iron reduction in the extremophile Acidithiobacillus ferrooxidans.Applied and environmental microbiology · 2013Article
- Cloning, expression and bioinformatics analysis of ATP sulfurylase from Acidithiobacillus ferrooxidans ATCC 23270 in Escherichia coli.Bioinformation · 2012Article
- Complete genome of Leptospirillum ferriphilum ML-04 provides insight into its physiology and environmental adaptation.Journal of microbiology (Seoul, Korea) · 2011Article
- Insights into the pH up-shift responsive mechanism of Acidithiobacillus ferrooxidans by microarray transcriptome profiling.Folia microbiologica · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAcidithiobacillus ferrooxidans is a gamma-proteobacterium that lives at pH2 and obtains energy by the oxidation of sulfur and iron. It is used in the biomining industry for the recovery of metals and is one of the causative agents of acid mine drainage. Effective tools for the study of its genetics and physiology are not in widespread use and, despite considerable effort, an understanding of its unusual physiology remains at a rudimentary level. Nearly complete genome sequences of A. ferrooxidans are available from two public sources and we have exploited this information to reconstruct aspects of its sulfur metabolism.
resultsTwo candidate mechanisms for sulfate uptake from the environment were detected but both belong to large paralogous families of membrane transporters and their identification remains tentative. Prospective genes, pathways and regulatory mechanisms were identified that are likely to be involved in the assimilation of sulfate into cysteine and in the formation of Fe-S centers. Genes and regulatory networks were also uncovered that may link sulfur assimilation with nitrogen fixation, hydrogen utilization and sulfur reduction. Potential pathways were identified for sulfation of extracellular metabolites that may possibly be involved in cellular attachment to pyrite, sulfur and other solid substrates.
conclusionsA bioinformatic analysis of the genome sequence of A. ferrooxidans has revealed candidate genes, metabolic process and control mechanisms potentially involved in aspects of sulfur metabolism. Metabolic modeling provides an important preliminary step in understanding the unusual physiology of this extremophile especially given the severe difficulties involved in its genetic manipulation and biochemical analysis.
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