ArticleMolecular and cellular biology2004
Targeted nuclear import of open reading frame 1 protein is required for in vivo retrotransposition of a telomere-specific non-long terminal repeat retrotransposon, SART1.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Identification of charged amino acids required for nuclear localization of human L1 ORF1 protein.Mobile DNA · 2019Article
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Both the Exact Target Site Sequence and a Long Poly(A) Tail Are Required for Precise Insertion of the 18S Ribosomal DNA-Specific Non-Long Terminal Repeat Retrotransposon R7Ag.Molecular and cellular biology · 2016Article
- Insertion of Retrotransposons at Chromosome Ends: Adaptive Response to Chromosome Maintenance.Frontiers in genetics · 2015Review
- Lost in translation: The biogenesis of non-LTR retrotransposon proteins.Mobile genetic elements · 2013Article
- Cell division promotes efficient retrotransposition in a stable L1 reporter cell line.Mobile DNA · 2013Article
- Non-long terminal repeat (non-LTR) retrotransposons: mechanisms, recent developments, and unanswered questions.Mobile DNA · 2010Article
- Characterization of the sequence specificity of the R1Bm endonuclease domain by structural and biochemical studies.Nucleic acids research · 2007Article
- Essential domains for ribonucleoprotein complex formation required for retrotransposition of telomere-specific non-long terminal repeat retrotransposon SART1.Molecular and cellular biology · 2006Article
- Eukaryotic translational coupling in UAAUG stop-start codons for the bicistronic RNA translation of the non-long terminal repeat retrotransposon SART1.Molecular and cellular biology · 2005Article
- Telomere-specific non-LTR retrotransposons and telomere maintenance in the silkworm, Bombyx mori.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
- Essential motifs in the 3' untranslated region required for retrotransposition and the precise start of reverse transcription in non-long-terminal-repeat retrotransposon SART1.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-long terminal repeat (non-LTR) retrotransposons, most of which carry two open reading frames (ORFs), are abundant mobile elements that are distributed widely among eukaryotes. ORF2 encodes enzymatic domains, such as reverse transcriptase, that are conserved in all retroelements, but the functional roles of ORF1 in vivo are little understood. We show with green fluorescent protein-ORF1 fusion proteins that the ORF1 proteins of SART1, a telomeric repeat-specific non-LTR retrotransposon in Bombyx mori, are transported into the nucleus to produce a dotted localization pattern. Nuclear localization signals N1 (RRKR) and N2 (PSKRGRG) at the N terminus and a highly basic region in the center of SART1 ORF1 are involved in nuclear import and the dotted localization pattern in the nucleus, respectively. An in vivo retrotransposition assay clarified that at least three ORF1 domains, N1/N2, the central basic domain, and CCHC zinc fingers are required for SART1 retrotransposition. The nuclear import activity of SART1 ORF1 makes it clear that the ORF1 proteins of non-LTR retrotransposons work mainly in the nucleus, in contrast to the cytoplasmic action of Gag proteins of LTR elements. The functional domains found here in SART1 ORF1 will be useful for developing a more efficient and target-specific LINE-based gene delivery vector.
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