ArticleMolecular and cellular biology2004
Activation of Syk protein tyrosine kinase in response to osmotic stress requires interaction with p21-activated protein kinase Pak2/gamma-PAK.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- The crosstalk between non-coding RNAs and oxidative stress in cancer progression.Genes & diseases · 2025Review
- Spleen Tyrosine Kinase phosphorylates VE-cadherin to cause endothelial barrier disruption in acute lung injury.The Journal of biological chemistry · 2023Article
- Syk Inhibitors: New Computational Insights into Their Intraerythrocytic Action inInternational journal of molecular sciences · 2020Article
- Identifying a kinase network regulating FGF14:Nav1.6 complex assembly using split-luciferase complementation.PloS one · 2015Article
- TAZ suppresses NFAT5 activity through tyrosine phosphorylation.Molecular and cellular biology · 2012Article
- PAK signaling in cancer.Cellular logistics · 2012Article
- Tyrosine phosphorylation of 3BP2 regulates B cell receptor-mediated activation of NFAT.The Journal of biological chemistry · 2009Article
- Pak protein kinases and their role in cancer.Cancer metastasis reviews · 2009Review
- Essential and unique roles of PIP5K-gamma and -alpha in Fcgamma receptor-mediated phagocytosis.The Journal of cell biology · 2009Article
- Analysis of conformational changes during activation of protein kinase Pak2 by amide hydrogen/deuterium exchange.The Journal of biological chemistry · 2008Article
- PAK is regulated by PI3K, PIX, CDC42, and PP2Calpha and mediates focal adhesion turnover in the hyperosmotic stress-induced p38 pathway.The Journal of biological chemistry · 2008Article
- Activation of Syk by protein kinase C-delta regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via tyrosine phosphorylation of RelA/p65.The Journal of biological chemistry · 2008Article
- Mechanosensitive activation of K+ channel via phospholipase C-induced depletion of phosphatidylinositol 4,5-bisphosphate in B lymphocytes.The Journal of physiology · 2007Article
- Inhibition of cap-dependent translation via phosphorylation of eIF4G by protein kinase Pak2.The EMBO journal · 2005Article
- Negative control of the Myc protein by the stress-responsive kinase Pak2.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
The p21-activated serine/threonine protein kinase Pak2/gamma-PAK and the nonreceptor type of protein tyrosine kinase Syk are known to be activated when the cells are exposed to osmotic stress. The purpose of the present study was to examine whether Pak2 and Syk functionally cooperate in cellular signaling. Cotransfection studies revealed that Pak2 associates with Syk in COS cells. The constitutively active form of Cdc42 increases the association of Pak2 with Syk. Pak2 coexpressed with an inactive form of Cdc42 or kinase-inactive Pak2 interacts to a lesser extent with Syk, suggesting that Pak2-Syk association is enhanced by Pak2 activation. Interaction with Pak2 enhances the intrinsic kinase activity of Syk. This is supported by in vitro studies showing that Pak2 phosphorylates and activates Syk. Treatment of cells with sorbitol to induce hyperosmolarity results in the translocation of Pak2 and Syk to the region surrounding the nucleus and in dramatic enhancement of their association. Furthermore, cotransfection of Pak2 and Syk leads to the activation of c-Jun N-terminal kinase (JNK) under hyperosmotic conditions. Pak2 short interfering RNA suppresses sorbitol-mediated activation of endogenous Syk and JNK, thus identifying a novel pathway for JNK activation by Cdc42. These results demonstrate that Pak2 and Syk positively cooperate to regulate cellular responses to stress.
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