ArticleJournal of virology2004
Alternative polyadenylation of adeno-associated virus type 5 RNA within an internal intron is governed by both a downstream element within the intron 3' splice acceptor and an element upstream of the P41 initiation site.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- The Expression and Function of the Small Nonstructural Proteins of Adeno-Associated Viruses (AAVs).Viruses · 2024Review
- Role of the membrane-associated accessory protein (MAAP) in adeno-associated virus (AAV) infection.Journal of virology · 2024Article
- Development of a Novel Recombinant Adeno-Associated Virus Production System Using Human Bocavirus 1 Helper Genes.Molecular therapy. Methods & clinical development · 2018Article
- Minute Virus of Canines NP1 Protein Governs the Expression of a Subset of Essential Nonstructural Proteins via Its Role in RNA Processing.Journal of virology · 2017Article
- Germline viral "fossils" guide in silico reconstruction of a mid-Cenozoic era marsupial adeno-associated virus.Scientific reports · 2016Article
- Productive life cycle of adeno-associated virus serotype 2 in the complete absence of a conventional polyadenylation signal.The Journal of general virology · 2015Article
- Highly divergent integration profile of adeno-associated virus serotype 5 revealed by high-throughput sequencing.Journal of virology · 2014Article
- The assembly-activating protein promotes capsid assembly of different adeno-associated virus serotypes.Journal of virology · 2011Article
- Internal polyadenylation of the parvovirus B19 precursor mRNA is regulated by alternative splicing.The Journal of biological chemistry · 2011Article
- Block to the production of full-length B19 virus transcripts by internal polyadenylation is overcome by replication of the viral genome.Journal of virology · 2008Article
- Distance-dependent processing of adeno-associated virus type 5 RNA is controlled by 5' exon definition.Journal of virology · 2007Article
- Two G-rich regulatory elements located adjacent to and 440 nucleotides downstream of the core poly(A) site of the intronless melanocortin receptor 1 gene are critical for efficient 3' end processing.Molecular and cellular biology · 2007Article
- Positive and negative effects of adenovirus type 5 helper functions on adeno-associated virus type 5 (AAV5) protein accumulation govern AAV5 virus production.Journal of virology · 2007Article
- Expression profiles of bovine adeno-associated virus and avian adeno-associated virus display significant similarity to that of adeno-associated virus type 5.Journal of virology · 2006Article
- Liver transduction with recombinant adeno-associated virus is primarily restricted by capsid serotype not vector genotype.Journal of virology · 2006Article
- The expression strategy of goose parvovirus exhibits features of both the Dependovirus and Parvovirus genera.Journal of virology · 2005Article
- Human circovirus TT virus genotype 6 expresses six proteins following transfection of a full-length clone.Journal of virology · 2005Article
- Comparison of the transcription profile of simian parvovirus with that of the human erythrovirus B19 reveals a number of unique features.Journal of virology · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Adeno-associated virus type 5 (AAV5) has a linear, single-stranded DNA genome of ca. 5 kb and an overlapping transcription profile featuring multiple promoters and a single intron in the center of the genome. Unlike the situation for the prototype AAV2, AAV5 RNAs transcribed from upstream promoters at map units 7 (P7) and 19 (P19), which encode the viral Rep proteins, are predominantly polyadenylated at a site within the intron [(pA)p]. RNAs generated from the AAV5 capsid gene promoter P41, which is only 78 nucleotides (nt) upstream of the intron donor, and 281 nt upstream of (pA)p, primarily readthrough (pA)p, are polyadenylated at a more distal site at the 3' end of the genome [(pA)d] and ultimately spliced. The intron contains the core sequences sufficient for polyadenylation at (pA)p, which is governed by a G/U-rich downstream element that overlaps with the intron 3' A2 splice acceptor. In addition, polyadenylation of P7- and P19-generated RNAs at (pA)p is influenced by an upstream element that lies 5' to the start of the P41 transcript. Our results also suggest that splicing and polyadenylation of P41-generated RNA can compete for the same pool of precursor pre-mRNA molecules. The cis-acting signals within the A2 3' splice site that govern polyadenylation and splicing of AAV5 RNAs must be optimized to program both (i) the levels of polyadenylation of P7- and P19-generated RNA at (pA)p required to generate the proper levels of the essential Rep proteins and (ii) the splicing of P41-generated RNAs to generate the proper ratio of capsid proteins during AAV5 infection.
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