ArticleBMC biology2003
TbAGO1, an argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei.
Article in BMC biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 88 citations in OpenAlex.
- Role of the nucleotide excision repair endonuclease XPF in the kinetoplastid parasite Trypanosoma brucei.Scientific reports · 2025Article
- Comparative structural insights and functional analysis for the distinct unbound states of Human AGO proteins.Scientific reports · 2025Article
- Current Status of Regulatory Non-Coding RNAs Research in the Tritryp.Non-coding RNA · 2022Review
- Article
- Mutations in PpAGO3 Lead to Enhanced Virulence ofFrontiers in microbiology · 2022Article
- AGO2 localizes to cytokinetic protrusions in a p38-dependent manner and is needed for accurate cell division.Communications biology · 2021Article
- Conserved chromosomal functions of RNA interference.Nature reviews. Genetics · 2020Review
- AGO unchained: Canonical and non-canonical roles of Argonaute proteins in mammals.Frontiers in bioscience (Landmark edition) · 2020Review
- RNA damage in biological conflicts and the diversity of responding RNA repair systems.Nucleic acids research · 2016Review
- Small RNA-Based Antiviral Defense in the Phytopathogenic Fungus Colletotrichum higginsianum.PLoS pathogens · 2016Article
- The Flagellar Arginine Kinase in Trypanosoma brucei Is Important for Infection in Tsetse Flies.PloS one · 2015Article
- Argonaute-1 functions as a mitotic regulator by controlling Cyclin B during Drosophila early embryogenesis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- The Comparative Genomics and Phylogenomics of Leishmania amazonensis Parasite.Evolutionary bioinformatics online · 2014Article
- Role of the Trypanosoma brucei HEN1 family methyltransferase in small interfering RNA modification.Eukaryotic cell · 2014Article
- Comparative transcriptome analysis of small noncoding RNAs in different stages of Trypanosoma brucei.RNA (New York, N.Y.) · 2013Article
- The structure and repertoire of small interfering RNAs in Leishmania (Viannia) braziliensis reveal diversification in the trypanosomatid RNAi pathway.Molecular microbiology · 2013Article
- Article
- Regulation of the cell division cycle in Trypanosoma brucei.Eukaryotic cell · 2012Review
- Comparative genomics reveals two novel RNAi factors in Trypanosoma brucei and provides insight into the core machinery.PLoS pathogens · 2012Article
- Genetic adaptation associated with genome-doubling in autotetraploid Arabidopsis arenosa.PLoS genetics · 2012Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA silencing processes are widespread in almost all eukaryotic organisms. They have various functions including genome protection, and the control of gene expression, development and heterochromatin formation. RNA interference (RNAi) is the post-transcriptional destruction of RNA, which is mediated by a ribonucleoprotein complex that contains, among several components, RNA helicases and Argonaute proteins. RNAi is functional in trypanosomes, protozoan parasites that separated very early from the main eukaryotic lineage and exhibit several intriguing features in terms of the control of gene expression. In this report, we investigated the functions of RNAi in Trypanosoma brucei.
resultsBy searching through genome databases, novel Argonaute-like proteins were identified in several protozoa that belong to the kinetoplastid order, a group of organisms that diverged early from the main eukaryotic lineage. T. brucei possesses two Argonaute-like genes termed TbAGO1 and TbPWI1. Dual transient transfection assays suggest that TbAGO1, but not TbPWI1, is involved in RNAi. The entire coding region of TbAGO1 was deleted by double gene knockout. TbAGO1-/- cells turned out to be completely resistant to RNAi generated either by transfected double-stranded RNA or by expression of an inverted repeat. TbAGO1-/- cells were viable but showed a dramatically reduced growth rate. This was probably due to defects in mitosis and abnormal chromosome segregation as revealed by in situ analysis. The RNAi and growth phenotypes were complemented by the inducible expression of a GFP::TbAGO1 fusion protein that revealed the cytoplasmic location of the protein.
conclusionsThe requirement of TbAGO1 for RNAi in trypanosomes demonstrates the evolutionary ancient involvement of Argonaute proteins in RNAi silencing processes. RNAi-deficient TbAGO1-/- cells showed numerous defects in chromosome segregation and mitotic spindle assembly. We propose a working hypothesis in which RNAi would be involved in heterochromatin formation at the centromere and therefore in chromosome segregation.
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