ArticleWorld journal of gastroenterology2003
Identification of alkA gene related to virulence of Shigella flexneri 2a by mutational analysis.
Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- The emerging role of recombineering in microbiology.Engineering microbiology · 2023Review
- Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics.Frontiers in microbiology · 2020Review
- A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a.Proteome science · 2010Article
- Use of the lambda Red-recombineering method for genetic engineering of Pantoea ananatis.BMC molecular biology · 2009Article
- Unraveling the secret lives of bacteria: use of in vivo expression technology and differential fluorescence induction promoter traps as tools for exploring niche-specific gene expression.Microbiology and molecular biology reviews : MMBR · 2005Review
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Authors and funding
8 authors.
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Abstract
aimIn vivo induced genes are thought to play an important role during infection of host. AlkA was identified as an in vivo-induced gene by in vivo expression technology (IVET), but its virulence in Shigella flexneri was not reported. The purpose of this study was to identify the role of alkA gene in the pathogenesis of S. flexneri.
methodsPCR was used to amplify alkA gene of S. flexneri 2a and fragment 028pKm. The fragment was then transformed into 2457T05 strain, a S flexneri 2a strain containing Red recombination system, which was constructed with a recombinant suicide plasmid pXLkd46. By in vivo homologous recombination, alkA mutants were obtained and verified by PCR and sequencing. Intracellular survival assay and virulence assay were used to test the intracellular survival ability in HeLa cell model and the virulence in mice lung infection model respectively.
resultsDeletion mutant of S. flexneri 2a alkA was successfully constructed by gamma Red recombination system. The mutant exhibited significant survival defects and much significant virulence defects in mice infection assay.
conclusionAlkA gene plays an important role in the infection of epithelial cells and is a virulent gene of Shigella spp.
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