Evidence map›Paper›PMID 14669320›Full record

ArticleWorld journal of gastroenterology2003

Distribution and expression of non-muscle myosin light chain kinase in rabbit livers.

Hua-Qing Zhu, Yuan Wang, Ruo-Lei Hu, Bin Ren, Qing Zhou, Zhi-Kui Jiang, Shu-Yu Gui

Open access · hybridAbstract read
In one paragraph

Article in World journal of gastroenterology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 78% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Hua-Qing ZhuLaboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Provience, China.
Yuan Wang
Ruo-Lei Hu
Bin Ren
Qing Zhou
Zhi-Kui Jiang
Shu-Yu Gui
Beth Israel Deaconess Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo study the distribution and expression of non-muscle myosin light chain kinase (nmMLCK) in rabbit livers.

methodsHuman nmMLCK N-terminal cDNA was amplified by polymerase chain reaction (PCR) and was inserted into pBKcmv to construct expression vectors. The recombinant plasmid was transformed into XL1-blue. Expression protein was induced by IPTG and then purified by SDS-PAGE and electroelution, which was used to prepare the polycolonal antibody to detect the distribution and expression of nmMLCK in rabbit livers with immunofluorescene techniques.

resultsThe polyclonal antibody was prepared, by which nmMLCK expression was detected and distributed mainly in peripheral hepatocytes.

conclusionnmMLCK can express in hepatocytes peripherally, and may play certain roles in the regulation of hepatic functions.

Indexed as

AnimalsBase SequenceCloning, MolecularEscherichia coliLiverMicroscopy, FluorescenceMolecular Sequence DataMyosin-Light-Chain KinasePolymerase Chain ReactionRabbitsMyosin-Light-Chain Kinasenon-muscle myosin light chain kinase, rabbit

Identifiers

PMID14669320
PMCPMC4612039
OpenAlexW2417878593

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.