ArticleMolecular biology of the cell2004
Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are sufficient for translocation of the ADP/ATP carrier across membranes.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 77 citations in OpenAlex.
- From the cytosol to the inner membrane: biogenesis of the mitochondrial carrier family.Protein science : a publication of the Protein Society · 2026Review
- Mitochondrial protein translocation machinery: From TOM structural biogenesis to functional regulation.The Journal of biological chemistry · 2022Review
- Altered Mitochondrial Protein Homeostasis and Proteinopathies.Frontiers in molecular neuroscience · 2022Review
- Mitochondrial Pharmacology of Dimebon (Latrepirdine) Calls for a New Look at its Possible Therapeutic Potential in Alzheimer's Disease.Aging and disease · 2018Review
- Engineering protocells: prospects for self-assembly and nanoscale production-lines.Life (Basel, Switzerland) · 2015Review
- Folding and biogenesis of mitochondrial small Tim proteins.International journal of molecular sciences · 2013Review
- Mitochondrial protein synthesis, import, and assembly.Genetics · 2012Article
- The transmembrane prolines of the mitochondrial ADP/ATP carrier are involved in nucleotide binding and transport and its biogenesis.The Journal of biological chemistry · 2012Article
- Protein translocation through Tom40: kinetics of peptide release.Biophysical journal · 2012Article
- Article
- Mitochondrial protein import and the genesis of steroidogenic mitochondria.Molecular and cellular endocrinology · 2011Review
- The Neurospora crassa TOB complex: analysis of the topology and function of Tob38 and Tob37.PloS one · 2011Article
- Interactions of mitochondrial presequence peptides with the mitochondrial outer membrane preprotein translocase TOM.Biophysical journal · 2010Article
- Roles of the Mdm10, Tom7, Mdm12, and Mmm1 proteins in the assembly of mitochondrial outer membrane proteins in Neurospora crassa.Molecular biology of the cell · 2010Article
- Review
- Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import.Molecular biology of the cell · 2009Article
- Physiological and transcriptional responses of Saccharomyces cerevisiae to zinc limitation in chemostat cultures.Applied and environmental microbiology · 2007Article
- The Tim9p/10p and Tim8p/13p complexes bind to specific sites on Tim23p during mitochondrial protein import.Molecular biology of the cell · 2007Article
- Hsp70 chaperones accelerate protein translocation and the unfolding of stable protein aggregates by entropic pulling.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Formation of membrane-bound ring complexes by prohibitins in mitochondria.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precursor proteins of the solute carrier family and of channel forming Tim components are imported into mitochondria in two main steps. First, they are translocated through the TOM complex in the outer membrane, a process assisted by the Tim9/Tim10 complex. They are passed on to the TIM22 complex, which facilitates their insertion into the inner membrane. In the present study, we have analyzed the function of the Tim9/Tim10 complex in the translocation of substrates across the outer membrane of mitochondria. The purified TOM core complex was reconstituted into lipid vesicles in which purified Tim9/Tim10 complex was entrapped. The precursor of the ADP/ATP carrier (AAC) was found to be translocated across the membrane of such lipid vesicles. Thus, these components are sufficient for translocation of AAC precursor across the outer membrane. Peptide libraries covering various substrate proteins were used to identify segments that are bound by Tim9/Tim10 complex upon translocation through the TOM complex. The patterns of binding sites on the substrate proteins suggest a mechanism by which portions of membrane-spanning segments together with flanking hydrophilic segments are recognized and bound by the Tim9/Tim10 complex as they emerge from the TOM complex into the intermembrane space.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.