ArticleGenetics2003
Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed.
- GSK-3β coordinates axonal microtubule organization through Shot and Tau.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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- Dendrite injury triggers neuroprotection in Drosophila models of neurodegenerative disease.Scientific reports · 2024Article
- piRNAs are regulators of metabolic reprogramming in stem cells.Nature communications · 2024Article
- Bruno 1/CELF regulates splicing and cytoskeleton dynamics to ensure correct sarcomere assembly in Drosophila flight muscles.PLoS biology · 2024Article
- Regulation of the evolutionarily conserved muscle myofibrillar matrix by cell type dependent and independent mechanisms.Nature communications · 2022Article
- Rbfox1 is required for myofibril development and maintaining fiber type-specific isoform expression inLife science alliance · 2022Article
- GFP-Tagged Protein Detection by Electron Microscopy Using a GBP-APEX Tool inFrontiers in cell and developmental biology · 2021Article
- The Drosophila FUS ortholog cabeza promotes adult founder myoblast selection by Xrp1-dependent regulation of FGF signaling.PLoS genetics · 2020Article
- Protein trap: a new Swiss army knife for geneticists?Molecular biology reports · 2020Review
- Efficient and flexible tagging of endogenous genes by homology-independent intron targeting.Genome research · 2019Article
- The neurobiological basis of sleep: Insights from Drosophila.Neuroscience and biobehavioral reviews · 2018Review
- In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae.Journal of visualized experiments : JoVE · 2018Article
- Review
- Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine.Cell stem cell · 2017Article
- Dissecting the Role of the Extracellular Matrix in Heart Disease: Lessons from the Drosophila Genetic Model.Veterinary sciences · 2017Review
- Mechanical tension and spontaneous muscle twitching precede the formation of cross-striated muscleDevelopment (Cambridge, England) · 2017Article
- CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells.Biology methods & protocols · 2017Article
- E2F function in muscle growth is necessary and sufficient for viability in Drosophila.Nature communications · 2016Article
- A conserved polybasic domain mediates plasma membrane targeting of Lgl and its regulation by hypoxia.The Journal of cell biology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
We describe a technique to tag Drosophila proteins with GFP at their native genomic loci. This technique uses a new, small P transposable element (the Wee-P) that is composed primarily of the green fluorescent protein (GFP) sequence flanked by consensus splice acceptor and splice donor sequences. We demonstrate that insertion of the Wee-P can generate GFP fusions with native proteins. We further demonstrate that GFP-tagged proteins have correct subcellular localization and can be expressed at near-normal levels. We have used the Wee-P to tag genes with a wide variety of functions, including transmembrane proteins. A genetic analysis of 12 representative fusion lines demonstrates that loss-of-function phenotypes are not caused by the Wee-P insertion. This technology allows the generation of GFP-tagged reagents on a genome-wide scale with diverse potential applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.