ArticleGenetics2003
Genetic modifier screens in Drosophila demonstrate a role for Rho1 signaling in ecdysone-triggered imaginal disc morphogenesis.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- A deficiency screen of the X chromosome for Rap1 GTPase dominant interacting genes in Drosophila border cell migration.G3 (Bethesda, Md.) · 2025Article
- Polycomb-mediated transgenerational epigenetic inheritance ofOpen biology · 2025Article
- Septate junction proteins are required for cell shape changes, actomyosin reorganization and cell adhesion during dorsal closure inFrontiers in cell and developmental biology · 2022Article
- Expanding the Junction: New Insights into Non-Occluding Roles for Septate Junction Proteins during Development.Journal of developmental biology · 2021Review
- The Broad Transcription Factor Links Hormonal Signaling, Gene Expression, and Cellular Morphogenesis Events DuringGenetics · 2020Article
- Apical and Basal Matrix Remodeling Control Epithelial Morphogenesis.Developmental cell · 2018Article
- Regulatory Mechanisms of Metamorphic Neuronal Remodeling Revealed Through a Genome-Wide Modifier Screen inGenetics · 2017Article
- Article
- Septate Junction Proteins Play Essential Roles in Morphogenesis Throughout Embryonic Development in Drosophila.G3 (Bethesda, Md.) · 2016Article
- Macroglobulin complement-related encodes a protein required for septate junction organization and paracellular barrier function in Drosophila.Development (Cambridge, England) · 2014Article
- The role of Drosophila Lamin C in muscle function and gene expression.Development (Cambridge, England) · 2010Article
- Sec61alpha is required for dorsal closure during Drosophila embryogenesis through its regulation of Dpp signaling.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Article
- Interaction between Drosophila bZIP proteins Atf3 and Jun prevents replacement of epithelial cells during metamorphosis.Development (Cambridge, England) · 2010Article
- uninflatable encodes a novel ectodermal apical surface protein required for tracheal inflation in Drosophila.Developmental biology · 2009Article
- Article
- A second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila.PloS one · 2009Article
- The transcriptional coactivator SAYP is a trithorax group signature subunit of the PBAP chromatin remodeling complex.Molecular and cellular biology · 2008Article
- Drosophila Myosin II, Zipper, is essential for ommatidial rotation.Developmental biology · 2007Article
- Mutational analysis of Stubble-stubbloid gene structure and function in Drosophila leg and bristle morphogenesis.Genetics · 2006Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila adult leg development provides an ideal model system for characterizing the molecular mechanisms of hormone-triggered morphogenesis. A pulse of the steroid hormone ecdysone at the onset of metamorphosis triggers the rapid transformation of a flat leg imaginal disc into an immature adult leg, largely through coordinated changes in cell shape. In an effort to identify links between the ecdysone signal and the cytoskeletal changes required for leg morphogenesis, we performed two large-scale genetic screens for dominant enhancers of the malformed leg phenotype associated with a mutation in the ecdysone-inducible broad early gene (br1). From a screen of >750 independent deficiency and candidate mutation stocks, we identified 17 loci on the autosomes that interact strongly with br1. In a complementary screen of approximately 112,000 F1 progeny of EMS-treated br1 animals, we recovered 26 mutations that enhance the br1 leg phenotype [E(br) mutations]. Rho1, stubbloid, blistered (DSRF), and cytoplasmic Tropomyosin were identified from these screens as br1-interacting genes. Our findings suggest that ecdysone exerts its effects on leg morphogenesis through a Rho1 signaling cascade, a proposal that is supported by genetic interaction studies between the E(br) mutations and mutations in the Rho1 signaling pathway. In addition, several E(br) mutations produce unexpected defects in midembryonic morphogenetic movements. Coupled with recent evidence implicating ecdysone signaling in these embryonic morphogenetic events, our results suggest that a common ecdysone-dependent, Rho1-mediated regulatory pathway controls morphogenesis during the two major transitions in the life cycle, embryogenesis and metamorphosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.