ArticleGenetics2003
Targeted gene expression using the GAL4/UAS system in the silkworm Bombyx mori.
Article in Genetics, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 162 citations in OpenAlex.
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- Cryptochrome-mediated integration of circadian rhythms into seasonal cycles in the silkwormFrontiers in physiology · 2026Review
- Article
- A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species.Scientific reports · 2024Article
- Possible Regulation of Larval Juvenile Hormone Titers inInsects · 2023Article
- Overexpression ofInternational journal of molecular sciences · 2023Article
- SericinInsects · 2023Article
- Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems.Scientific reports · 2022Article
- Establishment of a Novel Baculovirus-Silkworm Expression System.Microorganisms · 2022Article
- Genetic and viral approaches to record or manipulate neurons in insects.Current opinion in insect science · 2021Review
- Effects of transgenic silk materials that incorporate FGF-7 protein microcrystals on the proliferation and differentiation of human keratinocytes.FASEB bioAdvances · 2020Article
- Evaluating the probability of CRISPR-based gene drive contaminating another species.Evolutionary applications · 2020Article
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- The intronic promoter of Actin4 mediates high-level transgene expression mainly in the wing and epidermis of silkworms.Transgenic research · 2020Article
- Insights into the regulatory characteristics of silkworm fibroin gene promoters using a modified Gal4/UAS system.Transgenic research · 2019Article
- The expression of ecdysteroid UDP-glucosyltransferase enhances cocoon shell ratio by reducing ecdysteroid titre in last-instar larvae of silkworm, Bombyx mori.Scientific reports · 2018Article
- Optogenetic manipulation of medullary neurons in the locust optic lobe.Journal of neurophysiology · 2018Article
- An overview of functional genomic tools in deciphering insecticide resistance.Current opinion in insect science · 2018Review
- Melanoma antigen family A4 protein produced by transgenic silkworms induces antitumor immune responses.Experimental and therapeutic medicine · 2018Article
- Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: application to behavioral and developmental studies.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The silkworm Bombyx mori is one of the most well-studied insects in terms of both genetics and physiology and is recognized as the model lepidopteran insect. To develop an efficient system for analyzing gene function in the silkworm, we investigated the feasibility of using the GAL4/UAS system in conjunction with piggyBac vector-mediated germ-line transformation for targeted gene expression. To drive the GAL4 gene, we used two endogenous promoters that originated from the B. mori actin A3 (BmA3) and fibroin light-chain (FiL) genes and the artificial promoter 3xP3. GFP was used as the reporter. In initial tests of the function of the GAL4/UAS system, we generated transgenic animals that carried the UAS-GFP construct plus either BmA3-GAL4 or 3xP3-GAL4. GFP fluorescence was observed in the tissues of GFP-positive animals, in which both promoters drove GAL4 gene expression. Animals that possessed only the GAL4 gene or UAS-GFP construct did not show GFP fluorescence. In addition, as a further test of the ability of the GAL4/UAS system to drive tissue-specific expression we constructed FiL-GAL4 lines with 3xP3-CFP as the transformation marker. FiL-GAL4 x UAS-GFP crosses showed GFP expression in the posterior silk gland, in which the endogenous FiL gene is normally expressed. These results show that the GAL4/UAS system is applicable to B. mori and emphasize the potential of this system for controlled analyses of B. mori gene function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.