ArticleBMC evolutionary biology2003
Evolution of phage with chemically ambiguous proteomes.
Article in BMC evolutionary biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Advances in adaptive laboratory evolution applications forSynthetic and systems biotechnology · 2025Review
- Residue-Specific Incorporation of Noncanonical Amino Acids in Auxotrophic Hosts:Chemical reviews · 2025Review
- Effect of Non-Lethal Selection on Spontaneous Revertants of Frameshift Mutations: The Escherichia coli hisF CaseMicroorganisms · 2022Article
- Heat adaptation of phage T7 under an extended genetic code.Virus evolution · 2021Article
- Directed Evolution of theInternational journal of molecular sciences · 2021Article
- Evolving Bacterial Fitness with an Expanded Genetic Code.Scientific reports · 2018Article
- Future of the Genetic Code.Life (Basel, Switzerland) · 2017Review
- Article
- Coevolution Theory of the Genetic Code at Age Forty: Pathway to Translation and Synthetic Life.Life (Basel, Switzerland) · 2016Article
- Addicting diverse bacteria to a noncanonical amino acid.Nature chemical biology · 2016Article
- Overcoming Challenges in Engineering the Genetic Code.Journal of molecular biology · 2016Review
- Mutations enabling displacement of tryptophan by 4-fluorotryptophan as a canonical amino acid of the genetic code.Genome biology and evolution · 2014Article
- Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA.Nucleic acids research · 2010Article
- Article
- Causes for the intriguing presence of tRNAs in phages.Genome research · 2007Article
- Genetic code ambiguity confers a selective advantage on Acinetobacter baylyi.Journal of bacteriology · 2007Article
- A genetic code alteration generates a proteome of high diversity in the human pathogen Candida albicans.Genome biology · 2007Article
- Rewiring the severe acute respiratory syndrome coronavirus (SARS-CoV) transcription circuit: engineering a recombination-resistant genome.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Mistakes in translation don't translate into termination.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Inhibited cell growth and protein functional changes from an editing-defective tRNA synthetase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe widespread introduction of amino acid substitutions into organismal proteomes has occurred during natural evolution, but has been difficult to achieve by directed evolution. The adaptation of the translation apparatus represents one barrier, but the multiple mutations that may be required throughout a proteome in order to accommodate an alternative amino acid or analogue is an even more daunting problem. The evolution of a small bacteriophage proteome to accommodate an unnatural amino acid analogue can provide insights into the number and type of substitutions that individual proteins will require to retain functionality.
resultsThe bacteriophage Qbeta initially grows poorly in the presence of the amino acid analogue 6-fluorotryptophan. After 25 serial passages, the fitness of the phage on the analogue was substantially increased; there was no loss of fitness when the evolved phage were passaged in the presence of tryptophan. Seven mutations were fixed throughout the phage in two independent lines of descent. None of the mutations changed a tryptophan residue.
conclusionsA relatively small number of mutations allowed an unnatural amino acid to be functionally incorporated into a highly interdependent set of proteins. These results support the 'ambiguous intermediate' hypothesis for the emergence of divergent genetic codes, in which the adoption of a new genetic code is preceded by the evolution of proteins that can simultaneously accommodate more than one amino acid at a given codon. It may now be possible to direct the evolution of organisms with novel genetic codes using methods that promote ambiguous intermediates.
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