ArticleEukaryotic cell2003
Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein.
Article in Eukaryotic cell, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 71 citations in OpenAlex.
- Alterations to the yeast chromatin landscape during hypoxia.bioRxiv : the preprint server for biology · 2026Article
- Chromosome III Aneuploidy Enhances Ethanol Tolerance in Industrial Saccharomyces cerevisiae by Increasing the TUP1 Copy Number.Microbial biotechnology · 2025Article
- The influence of flocculation upon global gene transcription in a yeastMicrobial genomics · 2024Article
- Dynamic context-dependent regulation of auxin feedback signaling in synthetic gene circuits.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Role of ROX1, SKN7, and YAP6 Stress Transcription Factors in the Production of Secondary Metabolites inInternational journal of molecular sciences · 2022Article
- Transcriptional repressor Gal80 recruits corepressor complex Cyc8-Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon.Current genetics · 2022Article
- Article
- Genetic analysis argues for a coactivator function for the Saccharomyces cerevisiae Tup1 corepressor.Genetics · 2021Article
- Article
- Assessing regulatory features of the current transcriptional network of Saccharomyces cerevisiae.Scientific reports · 2020Article
- Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.Nucleic acids research · 2017Article
- The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene.Biochimica et biophysica acta · 2014Article
- Mapping small effect mutations in Saccharomyces cerevisiae: impacts of experimental design and mutational properties.G3 (Bethesda, Md.) · 2014Article
- Transcriptional regulation of yeast oxidative phosphorylation hypoxic genes by oxidative stress.Antioxidants & redox signaling · 2013Article
- The glucose signaling network in yeast.Biochimica et biophysica acta · 2013Review
- Mth1 regulates the interaction between the Rgt1 repressor and the Ssn6-Tup1 corepressor complex by modulating PKA-dependent phosphorylation of Rgt1.Molecular biology of the cell · 2013Article
- Activator and repressor functions of the Mot3 transcription factor in the osmostress response of Saccharomyces cerevisiae.Eukaryotic cell · 2013Article
- Systematic measurement of transcription factor-DNA interactions by targeted mass spectrometry identifies candidate gene regulatory proteins.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Shields up: the Tup1-Cyc8 repressor complex blocks coactivator recruitment.Genes & development · 2011Article
- The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein.Genes & development · 2011Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Tup1-Ssn6 general repression complex in Saccharomyces cerevisiae represses a wide variety of regulons. Regulon-specific DNA binding proteins recruit the repression complex, and their synthesis, activity, or localization controls the conditions for repression. Rox1 is the hypoxic regulon-specific protein, and a second DNA binding protein, Mot3, augments repression at tightly controlled genes. We addressed the requirements for Tup1-Ssn6 recruitment to two hypoxic genes, ANB1 and HEM13, by using chromatin immunoprecipitation assays. Either Rox1 or Mot3 could recruit Ssn6, but Tup1 recruitment required Ssn6 and Rox1. We also monitored events during derepression. Rox1 and Mot3 dissociated from DNA quickly, accounting for the rapid accumulation of ANB1 and HEM13 RNAs, suggesting a simple explanation for induction. However, Tup1 remained associated with these genes, suggesting that the localization of Tup1-Ssn6 is not the sole determinant of repression. We could not reproduce the observation that deletion of the Tup1-Ssn6-interacting protein Cti6 was required for induction. Finally, Tup1 is capable of repression through a chromatin-dependent mechanism, the positioning of a nucleosome over the TATA box, or a chromatin-independent mechanism. We found that the rate of derepression was independent of the positioned nucleosome and that the TATA binding protein was excluded from ANB1 even in the absence of the positioned nucleosome. The mediator factor Srb7 has been shown to interact with Tup1 and to play a role in repression at several regulons, but we found that significant levels of repression remained in srb7 mutants even when the chromatin-dependent repression mechanism was eliminated. These findings suggest that the repression of different regulons or genes may invoke different mechanisms.
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