ArticleThe Biochemical journal2004
Characterization of a cis-acting regulatory element in the protein-coding region of human dihydrofolate reductase mRNA.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Antithymidylate resistance enables transgene selection and cell survival for T cells in the presence of 5-fluorouracil and antifolates.Gene therapy · 2016Article
- RNA-Binding Proteins in Trichomonas vaginalis: Atypical Multifunctional Proteins.Biomolecules · 2015Review
- Dihydrofolate Reductase and Thymidylate Synthase Transgenes Resistant to Methotrexate Interact to Permit Novel Transgene Regulation.The Journal of biological chemistry · 2015Article
- Regulation of luteinizing hormone receptor expression by an RNA binding protein: role of ERK signaling.The Indian journal of medical research · 2014Review
- A var gene upstream element controls protein synthesis at the level of translation initiation in Plasmodium falciparum.PloS one · 2014Article
- Frac-seq reveals isoform-specific recruitment to polyribosomes.Genome research · 2013Article
- Enhanced degradation of dihydrofolate reductase through inhibition of NAD kinase by nicotinamide analogs.Molecular pharmacology · 2013Article
- Structure, function and regulation of gonadotropin receptors - a perspective.Molecular and cellular endocrinology · 2012Review
- Molecular regulation of gonadotropin receptor expression: relationship to sterol metabolism.Molecular and cellular endocrinology · 2010Review
- Identification and characterization of proteins that selectively interact with the LHR mRNA binding protein (LRBP) in rat ovaries.Biochimica et biophysica acta · 2010Article
- Species-specific differences in translational regulation of dihydrofolate reductase.Molecular pharmacology · 2009Article
- Tetrahydrobiopterin recycling, a key determinant of endothelial nitric-oxide synthase-dependent signaling pathways in cultured vascular endothelial cells.The Journal of biological chemistry · 2009Article
- HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.The Journal of infectious diseases · 2009Article
- miR-192 Regulates dihydrofolate reductase and cellular proliferation through the p53-microRNA circuit.Clinical cancer research : an official journal of the American Association for Cancer Research · 2008Article
- Endothelial dihydrofolate reductase: critical for nitric oxide bioavailability and role in angiotensin II uncoupling of endothelial nitric oxide synthase.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies have shown that human DHFR (dihydrofolate reductase), in addition to its critical role in DNA biosynthesis, functions as an RNA-binding protein. The interaction between DHFR and its own mRNA results in translational repression. In this study, we characterized the cis-acting elements on human DHFR mRNA that are required for the DHFR mRNA-DHFR protein interaction. Using a series of gel-shift and nitrocellulose filter-binding assays, a 164 nt RNA sequence, corresponding to nt 401-564, was identified within the coding region that binds to DHFR protein with an affinity similar to that of full-length DHFR mRNA. To document in vivo biological activity, various DHFR sequences contained within the coding region were cloned on to the 5' end of a luciferase reporter plasmid, and transient transfection experiments were performed using human colon cancer RKO cells. In cells transfected with p644/DHFR:401-564, luciferase activity was decreased by 50% when compared with cells transfected with the p644 plasmid alone. Luciferase mRNA levels were identical under each of these conditions, as determined by Northern-blot analysis. In cells transfected with p644/DHFR:401-564, luciferase activity was restored to almost 100% of control when cells were treated with the antifolate analogue methotrexate or with a short-interfering RNA targeting DHFR mRNA. These findings provide evidence that the DHFR 401-564 sequence is a DHFR-response element. In vitro and in vivo studies further localized this cis-element to an 82 nt sequence corresponding to nt 401-482. This work provides new insights into critical elements that mediate RNA-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.