ArticleEuropean biophysics journal : EBJ2004
The effect of diffusion, depolymerization and nucleation promoting factors on actin gel growth.
Article in European biophysics journal : EBJ, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 50 citations in OpenAlex.
- Multi-condensate state as a functional strategy to optimize the cell signaling output.Nature communications · 2024Article
- Multi-condensate state as a functional strategy to optimize the cell signaling output.bioRxiv : the preprint server for biology · 2024Article
- Liquid-like VASP condensates drive actin polymerization and dynamic bundling.Nature physics · 2023Article
- Myo1c facilitates G-actin transport to the leading edge of migrating endothelial cells.The Journal of cell biology · 2012Article
- A model actin comet tail disassembling by severing.Physical biology · 2011Article
- Effects of molecular-scale processes on observable growth properties of actin networks.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- An experimental and computational study of the effect of ActA polarity on the speed of Listeria monocytogenes actin-based motility.PLoS computational biology · 2009Article
- Models for actin polymerization motors.Journal of mathematical biology · 2009Review
- Reconstitution of an actin cortex inside a liposome.Biophysical journal · 2009Article
- Protein localization by actin treadmilling and molecular motors regulates stereocilia shape and treadmilling rate.Biophysical journal · 2008Article
- New proposed mechanism of actin-polymerization-driven motility.Biophysical journal · 2008Article
- Arp2/3 controls the motile behavior of N-WASP-functionalized GUVs and modulates N-WASP surface distribution by mediating transient links with actin filaments.Biophysical journal · 2008Article
- Relaxing the actin cytoskeleton for adhesion and movement with Ena/VASP.The Journal of cell biology · 2008Review
- Deformations in actin comets from rocketing beads.Biophysical journal · 2006Article
- Diffusion rate limitations in actin-based propulsion of hard and deformable particles.Biophysical journal · 2006Article
- Dynamics of membranes driven by actin polymerization.Biophysical journal · 2006Article
- Bacterial shape and ActA distribution affect initiation of Listeria monocytogenes actin-based motility.Biophysical journal · 2005Article
- Stress release drives symmetry breaking for actin-based movement.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotic cells, localized actin polymerization is able to deform the plasma membrane and push the cell forward. Depolymerization of actin filaments and diffusion of actin monomers ensure the availability of monomers at sites of polymerization, and therefore these processes must play an active role in cellular actin dynamics. Here we reveal experimental evidence that actin gel growth can be limited by monomer diffusion, consistent with theoretical predictions. We study actin gels formed on beads coated with ActA (and ActA fragments), the bacterial factor responsible for actin-based movement of Listeria monocytogenes. We observe a saturation of gel thickness with increasing bead radius, the signature of diffusion control. Data analysis using an elastic model of actin gel growth gives an estimate of 2x10(-8) cm(-2) s(-1) for the diffusion coefficient of actin monomers through the gel, ten times less than in buffer, and in agreement with literature values in bulk cytoskeleton, providing corroboration of our model. The depolymerization rate of actin filaments and the elastic modulus of the gel are also evaluated. Furthermore, we qualitatively examine the different actin gels produced when ActA fragments interact with either VASP or the Arp2/3 complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.