ArticleProceedings of the National Academy of Sciences of the United States of America2003
Marker succession during the development of keratinocytes from cultured human embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications.Stem cell reviews and reports · 2024Pooled it
- Multiple cell types guided by neurocytes orchestrate horn bud initiation in dairy goats.Genetics, selection, evolution : GSE · 2025Article
- Clinical, Dermoscopic, and Molecular Features of Acantholytic Squamous Cell Carcinoma: A Systematic Review.Cancers · 2024Review
- Cis-regulatory chromatin loops analysis identifies GRHL3 as a master regulator of surface epithelium commitment.Science advances · 2022Article
- Reversibly immortalized keratinocytes (iKera) facilitate re-epithelization and skin wound healing: Potential applications in cell-based skin tissue engineering.Bioactive materials · 2022Article
- Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells.Regenerative therapy · 2021Article
- The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma.Cancers · 2021Article
- Ex vivo gene modification therapy for genetic skin diseases-recent advances in gene modification technologies and delivery.Experimental dermatology · 2021Review
- A Single-cell Transcriptome Atlas of Cashmere Goat Hair Follicle Morphogenesis.Genomics, proteomics & bioinformatics · 2021Article
- Generation of Skin Organoids: Potential Opportunities and Challenges.Frontiers in cell and developmental biology · 2021Review
- Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.International journal of biological sciences · 2020Article
- Protein biomarkers of neural system.Journal of otology · 2019Review
- Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling.PLoS genetics · 2019Article
- Bioapplications of Bacterial Cellulose Polymers Conjugated with Resveratrol for Epithelial Defect Regeneration.Polymers · 2019Article
- Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane.Stem cell research & therapy · 2019Article
- A novel method to reconstruct epithelial tissue using high-purity keratinocyte lineage cells induced from human embryonic stem cells.Cell cycle (Georgetown, Tex.) · 2019Article
- Umbilical Cord Tissue Derived Mesenchymal Stem Cells can Differentiate into Skin Cells.Open life sciences · 2018Article
- Cell therapy for severe burn wound healing.Burns & trauma · 2018Review
- An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs.Stem cell reports · 2017Article
- Molecular and cytoskeletal regulations in epidermal development.Seminars in cell & developmental biology · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Human embryonic stem cells injected into scid mice produce nodules containing differentiated somatic tissues. From the trypsinized cells of such a nodule, we have recovered keratinocytes that can be grown in cell culture. The method of recovery is sensitive enough to detect small numbers of keratinocytes formed in the nodule, but for purposes of analysis, it is preferable to study the development of the entire keratinocyte lineage in culture. The principle of our analysis is the successive appearance of markers, including transcription factors with considerable specificity for the keratinocyte (p63 and basonuclin) and differentiation markers characteristic of its final state (keratin 14 and involucrin). We have determined the order of marker succession during the time- and migration-dependent development of keratinocytes from single embryoid bodies in cell culture. Of the markers we have examined, p63 was the earliest to appear in the keratinocyte lineage. The successive accumulation of later markers provides increasing certainty of emergence of the definitive keratinocyte.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.