ArticleJournal of clinical microbiology2003
rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria.
Article in Journal of clinical microbiology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 261 papers, 3 of them syntheses that pooled it.
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Who cites it
261 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Diagnostic performance of MALDI-TOF MS for non-tuberculous mycobacteria: A systematic review and meta-analysis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Pooled it
- Treatment of Nontuberculous Mycobacterial Pulmonary Disease: An Official ATS/ERS/ESCMID/IDSA Clinical Practice Guideline.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Guideline
- Treatment of nontuberculous mycobacterial pulmonary disease: an official ATS/ERS/ESCMID/IDSA clinical practice guideline.The European respiratory journal · 2020Guideline
- Diagnosis of Mycobacteriosis in Cultured Hybrid Tilapia (Oreochromis spp.) in Trinidad and Tobago.Journal of fish diseases · 2026Article
- Disinfection and damage of nontuberculous mycobacteria by different UV wavelengths.Applied and environmental microbiology · 2026Article
- Utility of RGM medium for recovery ofIranian journal of microbiology · 2026Article
- Are you for real,ASM case reports · 2026Article
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- Severe pneumonia and bloodstream dissemination caused by Mycobacterium houstonense in a patient with achalasia: clinical importance of genospecies typing.BMC infectious diseases · 2026Article
- Comorbidity profiles of non-tuberculous mycobacteria infections in China: 12 years' experience.Microbiology spectrum · 2026Article
- Species distribution of nontuberculous mycobacteria isolated from respiratory specimens at a tertiary care hospital in South Korea, 2017-2022.Microbiology spectrum · 2025Article
- Consequences of Climate Change on the Emergence of Pathogenic, Environmentally Acquired Nontuberculous Mycobacteria.Open forum infectious diseases · 2025Article
- Microbiological Quality and Presence of Clinically Relevant Nontuberculous Mycobacteria in Purified Water from Vending Machines in Michoacan, Mexico.Pathogens (Basel, Switzerland) · 2025Article
- Mycobacterium hainanense sp. nov. represents an emerging nontuberculous Mycobacterium associated with chronic pulmonary disease.Scientific reports · 2025Article
- Culture and real-time quantitative PCR to detect environmental nontuberculous mycobacteria in a clinical care center.Total environment microbiology · 2025Article
- Analysis of relative genes expression and mutation of pstB and efpA efflux pumps in Mycobacterium simiae isolates from suspected tuberculosis patients by using quantitative Real-time PCR.BMC microbiology · 2025Article
- Diagnostics, resistance and clinical relevance of non-tuberculous mycobacteria unidentified at the species level by line probe assays: a bi-national study.Annals of clinical microbiology and antimicrobials · 2025Article
- First isolation of Mycobacterium brisbanense from humans in the Americas.Memorias do Instituto Oswaldo Cruz · 2025Article
- Comparative evaluation of MALDI-ToF mass spectrometry and Sanger sequencing of theFrontiers in cellular and infection microbiology · 2025Article
- Diagnosis of nontuberculous mycobacterial infections using genomics and artificial intelligence-machine learning approaches: scope, progress and challenges.Frontiers in microbiology · 2025Review
201 more citing papers are in PubMed but not listed here.
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3 authors.
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Abstract
Nonpigmented and late-pigmenting rapidly growing mycobacteria (RGM) are increasingly isolated in clinical microbiology laboratories. Their accurate identification remains problematic because classification is labor intensive work and because new taxa are not often incorporated into classification databases. Also, 16S rRNA gene sequence analysis underestimates RGM diversity and does not distinguish between all taxa. We determined the complete nucleotide sequence of the rpoB gene, which encodes the bacterial beta subunit of the RNA polymerase, for 20 RGM type strains. After using in-house software which analyzes and graphically represents variability stretches of 60 bp along the nucleotide sequence, our analysis focused on a 723-bp variable region exhibiting 83.9 to 97% interspecies similarity and 0 to 1.7% intraspecific divergence. Primer pair Myco-F-Myco-R was designed as a tool for both PCR amplification and sequencing of this region for molecular identification of RGM. This tool was used for identification of 63 RGM clinical isolates previously identified at the species level on the basis of phenotypic characteristics and by 16S rRNA gene sequence analysis. Of 63 clinical isolates, 59 (94%) exhibited <2% partial rpoB gene sequence divergence from 1 of 20 species under study and were regarded as correctly identified at the species level. Mycobacterium abscessus and Mycobacterium mucogenicum isolates were clearly distinguished from Mycobacterium chelonae; Mycobacterium mageritense isolates were clearly distinguished from "Mycobacterium houstonense." Four isolates were not identified at the species level because they exhibited >3% partial rpoB gene sequence divergence from the corresponding type strain; they belonged to three taxa related to M. mucogenicum, Mycobacterium smegmatis, and Mycobacterium porcinum. For M. abscessus and M. mucogenicum, this partial sequence yielded a high genetic heterogeneity within the clinical isolates. We conclude that molecular identification by analysis of the 723-bp rpoB sequence is a rapid and accurate tool for identification of RGM.
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